Electrospun scaffolds are emerging as extracellular matrix (ECM)mimicking structures for tissue engineering thanks to their nanofibrous architecture. For the development of suitable electrospun scaffolds for bone tissue engineering, the addition of inorganic components has been implemented with the aim to confer important bioactivity like osteoinduction, osteointegration, and cell adhesion to the scaffolds. In this context, we propose a tricomponent electrospun scaffold composed of poly(d,l‐lactide), gelatin and RKKP glass‐ceramics. The bioactive RKKP glass‐ceramic system has attracted interest, due to the presence of ions such as La³⁺ and Ta⁵⁺, which turned out to be valuable as growth supporting agents for bones. In this work, RKKP glass‐ceramics were embedded inside the microfibers of electrospun scaffolds and the structural and biological properties were investigated. Our results showed that the glass‐ceramic microparticles were uniformly distributed in the fibrous structure of the scaffold. Furthermore, the glass‐ceramics promoted biomineralization of the scaffolds and improved cell viability and osteogenic differentiation. The mineralized layer formed on RKKP‐containing scaffolds after incubation in simulated body fluid medium has been shown to be hydroxyapatite by Raman spectroscopy and X‐ray diffraction. The results on differentiation studies of canine adipose‐derived mesenchymal stem cells grown on the electrospun scaffolds suggest that on varying the content of RKKP in the scaffold, it is possible to drive the differentiation toward chondrogenic or osteogenic commitment. The presence of ions, like La³⁺ and Ta⁵⁺, in the RKKP embedded polymeric composite scaffolds could play a role in supporting cell growth and promoting differentiation.

中文翻译：

---

用于骨组织工程的静电纺聚（d，l-丙交酯）/明胶/玻璃陶瓷三组分纳米纤维支架。

由于其纳米纤维结构，电纺支架正在成为组织工程的细胞外基质 (ECM) 模拟结构。为了开发适合骨组织工程的静电纺丝支架，已经实施了添加无机成分的目的是赋予支架重要的生物活性，如骨诱导、骨整合和细胞粘附。在这种情况下，我们提出了一种由聚（d,l-丙交酯）、明胶和 RKKP 玻璃陶瓷组成的三组分电纺支架。由于 La ³⁺和 Ta ⁵⁺等离子的存在，生物活性 RKKP 微晶玻璃系统引起了人们的兴趣，结果证明它是有价值的骨骼生长支持剂。在这项工作中，将 RKKP 玻璃陶瓷嵌入电纺支架的微纤维内，并研究了其结构和生物学特性。我们的结果表明微晶玻璃微粒均匀分布在支架的纤维结构中。此外，玻璃陶瓷促进了支架的生物矿化，提高了细胞活力和成骨分化。在模拟体液介质中孵育后，在含 RKKP 的支架上形成的矿化层已通过拉曼光谱和 X 射线衍射显示为羟基磷灰石。在电纺支架上生长的犬脂肪源性间充质干细胞的分化研究结果表明，通过改变支架中 RKKP 的含量，有可能推动向软骨形成或成骨承诺的分化。离子的存在，如 La³⁺和Ta ⁵⁺，在RKKP 嵌入的聚合物复合支架中可以起到支持细胞生长和促进分化的作用。