Polyacrylamide gel electrophoresis (PAGE) is a widely used technique in protein separations. In this study, the effects of pre‐electrophoresis (i.e., exposing polyacrylamide gels to an electrical field prior to loading samples) on the separation of a mixture of 10 standard proteins was investigated. Three different gel concentrations (based on amounts of acrylamide and bis‐acrylamide, always at a 37.5:1 ratio) relative to the volume of the gel solution were tested: 6%, 9%, and 12%. For each concentration, multiple slab gels were subjected to pre‐electrophoresis prior to separation of proteins, while multiple other (i.e., companion) slab gels were used but without applying the pre‐electrophoresis technique. Separation results were investigated, showing that the application of the pre‐electrophoresis technique had a significant effect on separations particularly for 6% and 9% gels.

中文翻译：

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电泳前对聚丙烯酰胺凝胶中蛋白质分离的影响

聚丙烯酰胺凝胶电泳（PAGE）是蛋白质分离中广泛使用的技术。在这项研究中，研究了电泳前（即在加载样品前将聚丙烯酰胺凝胶暴露于电场中）对10种标准蛋白混合物分离的影响。相对于凝胶溶液的体积，测试了三种不同的凝胶浓度（基于丙烯酰胺和双丙烯酰胺的量，始终为37.5：1的比例）：6％，9％和12％。对于每种浓度，在分离蛋白质之前先对多个平板凝胶进行预电泳，同时使用多个其他（即，同伴）平板凝胶，但不应用电泳前技术。研究了分离结果，