In this study, Ti³⁺ self-doped TiO₂ decorated with metallic copper photocatalyst (marked as Cu/Ti³⁺-TiO₂) was successfully prepared by a novel method. The ESR (Electron Spin Resonance) analysis indicated that the synthesized sample contained Ti³⁺. The TEM (transmission electron microscopy) images showed that the TiO₂ nanoparticles were modified with nano copper. The as-synthesized photocatalyst was found to exhibit markedly enhanced photocatalytic inactivation of E. coli under visible light irradiation compared with pure TiO₂, especially in the case of the 10% Cu/Ti³⁺-TiO₂ sample. The photodisinfection effect of the 10% Cu/Ti³⁺-TiO₂ sample was further confirmed using five agricultural pathogenic fungal spores. The photocatalytic inactivation effect against F. graminearum and Botryosphaeria dothidea spores was certified by fluorescent staining of CFDA-AM (the acetoxymethyl ester of the esterase substrate 5-carboxyfluorescein diacetate)/ PI (Propidium Iodide). In addition, fluorescent staining by H₂DCFDA demonstrated that the intracellular ROS (reactive oxygen species) of the F. graminearum macroconidia was increased after treatment by the photocatalysis.

本研究成功地通过新颖的方法制备了装饰有金属铜光催化剂的Ti ³⁺自掺杂TiO ₂（标记为Cu / Ti ³⁺ -TiO ₂）。ESR（电子自旋共振）分析表明合成的样品含有Ti ³⁺。TEM（透射电子显微镜）图像显示，TiO ₂纳米颗粒被纳米铜改性。发现与纯TiO ₂相比，合成后的光催化剂在可见光照射下对大肠杆菌表现出明显增强的光催化失活，特别是在10％Cu / Ti ³⁺ -TiO _{2的情况下}样品。使用5种农业致病真菌孢子进一步证实了10％Cu / Ti ³⁺ -TiO ₂样品的光消毒作用。通过CFDA-AM（酯酶底物5-羧基荧光素二乙酸酯的乙酰氧基甲基酯）/ PI（碘化丙啶）的荧光染色，证明了对禾谷镰孢和灰霉病菌孢子的光催化失活作用。另外，通过H ₂ DCFDA的荧光染色表明，通过光催化处理后，禾谷镰刀菌大分生孢子的细胞内ROS（活性氧种类）增加。