Sea cucumbers attracted increasing interest due to its nutritional functions. Collagen is the most important structural biomacromolecule in sea cucumber body wall, and is highly related to the textual properties and food quality of sea cucumber. In this study, the type of constituent collagens of sea cucumber collagen fibrils was investigated, employing a commercially important species Apostichopus japonicus as the material. Proteomics and bioinformatics analysis revealed that collagen fibrils of A. japonicas are heterotypic. Two clade A and one clade B fibrillar collagens and two FACIT collagens were identified from the fibrils. Besides, the heterogeneity was also revealed in the pepsin-solubilized collagen (PSC) of A. japonicus by using the proteomics strategy. It implied that the previous conclusions on the type of sea cucumber collagen deduced from SDS-PAGE analysis should be rechecked. The results provided novel insight into the composition of sea cucumber collagen fibrils.

中文翻译：

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海参（Apostichopus japonicus）的胶原蛋白原纤维是异型的。

海参由于其营养功能而引起了越来越多的兴趣。胶原蛋白是海参体壁中最重要的结构性生物大分子，并且与海参的质地和食品质量高度相关。在这项研究中，以商业上重要的日本刺槐为材料，研究了海参胶原蛋白原纤维的胶原蛋白类型。蛋白质组学和生物信息学分析表明，粳稻的胶原纤维是异型的。从原纤维中鉴定出两个进化枝A和一个进化枝B的原纤维胶原和两个FACIT胶原。此外，采用蛋白质组学方法，还可以发现日本曲霉胃蛋白酶溶解的胶原蛋白（PSC）具有异质性。这暗示着应重新检查先前从SDS-PAGE分析得出的关于海参胶原蛋白类型的结论。结果为海参胶原蛋白原纤维的组成提供了新颖的见解。