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Ultrasensitive electrochemiluminescence biosensing platform for miRNA-21 and MUC1 detection based on dual catalytic hairpin assembly
Analytica Chimica Acta ( IF 6.2 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.aca.2020.01.034
Jiyang Li , Jingju Liu , Yanni Bi , Mimi Sun , Jing Bai , Ming Zhou

Multi-target detection has been widely applied for the sensitive measurement of cancer-related biomarkers; however, the design and application of single platforms for diverse target detection are still challenging. Herein, a robust and sensitive electrochemiluminescence (ECL) biosensing platform was constructed for the measurement of microRNA-21 (miRNA-21) and mucin 1 (MUC1) based on dual catalytic hairpin assembly (DCHA). The catalytic hairpin assembly (CHA) process (Cycle I) was initiated by the target miRNA-21 to introduce abundant CdS:Mn quantum dots (CdS:Mn QDs) on the electrode surface, leading to a considerable ECL response and the sensitive detection of miRNA-21 with a limit of detection as low as 11 aM. Subsequently, the second CHA process (Cycle II) was triggered by the MUC1-aptamer complex, which allowed copious amounts of Au nanoparticles (AuNPs) to approach the CdS:Mn QDs. A decreased ECL signal was obtained due to the ECL resonance energy transfer (ECL-RET) effect between the CdS:Mn QDs and AuNPs; meanwhile, MUC1 was sensitively detected with a limit of detection of 0.40 fg mL-1. This single sensing platform achieved dual cancer-related biomarker detection, which could provide a rational approach for future clinical analyse.

中文翻译:

基于双催化发夹组装的用于miRNA-21和MUC1检测的超灵敏电化学发光生物传感平台

多靶点检测已广泛应用于癌症相关生物标志物的灵敏测量;然而,用于不同目标检测的单一平台的设计和应用仍然具有挑战性。在此,基于双催化发夹组装 (DCHA) 构建了一个强大且灵敏的电化学发光 (ECL) 生物传感平台,用于测量 microRNA-21 (miRNA-21) 和粘蛋白 1 (MUC1)。催化发夹组装 (CHA) 过程 (Cycle I) 由目标 miRNA-21 启动以在电极表面引入丰富的 CdS:Mn 量子点 (CdS:Mn QDs),导致相当大的 ECL 响应和灵敏的检测miRNA-21 的检测限低至 11 aM。随后,第二个 CHA 过程(周期 II)由 MUC1-适配体复合物触发,这允许大量的 Au 纳米粒子 (AuNPs) 接近 CdS:Mn QD。由于 CdS:Mn QDs 和 AuNPs 之间的 ECL 共振能量转移 (ECL-RET) 效应,获得了降低的 ECL 信号;同时,MUC1 被灵敏地检测到,检测限为 0.40 fg mL-1。这种单一传感平台实现了双重癌症相关生物标志物检测,可为未来的临床分析提供合理的方法。
更新日期:2020-04-01
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