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Simultaneous separation and concentration of neutral analytes by cyclodextrin assisted sweeping-micellar electrokinetic chromatography
Analytica Chimica Acta ( IF 6.2 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.aca.2020.01.037
Li-Qing Peng , Xin Dong , Xiao-Ting Zhen , Juan Yang , Yan Chen , Shu-Ling Wang , Tian Xie , Jun Cao

An on-line cyclodextrin assisted sweeping-micellar electrokinetic chromatography (CD assisted sweeping-MEKC) was developed for the simultaneous separation and concentration of four neutral analytes (erianin, dendrophenol, naringenin and scoparone) in Dendrobium officinale Kimura et Migo (D. officinale). The D. officinale was directly determined by this on-line stacking method after simple extraction and dilution. The optimized background solution (BGS) was 50 mM phosphoric acid (PA) containing 100 mM SDS and 30% (v/v) methanol. The best separation and concentration performance of analytes dissolved in 90 mM CD and 100 mM PA was achieved in a short analysis time when injected at 50 mbar for 100 s. Compared with conventional sweeping-MEKC and MEKC method, significant improvement in enrichment efficiency was achieved by using this proposed method. A series of validation studies of the present method was performed under the optimal conditions. Good linearities were obtained with the correlation coefficients in the range of 0.994-0.999, the detection limits were ranged from 13 to 40 ng/mL. Sensitivity enhancement factors (SEFs) were in the range of 28.5-46.8 compared with traditional injection (injection time 3 s). Therefore, the proposed method was successfully applied for the separation and concentration of neutral analytes in real samples.

中文翻译:

环糊精辅助扫描胶束电动色谱法同时分离和浓缩中性分析物

开发了一种在线环糊精辅助清扫-胶束电动色谱(CD 辅助清扫-MEKC),用于同时分离和浓缩铁皮石斛(Dendrobium officinale Kimura et Migo,D. officinale)中的四种中性分析物(艾叶素、树果酚、柚皮素和scoparone) . 通过简单的提取和稀释后,通过这种在线叠加法直接测定铁皮石斛。优化的背景溶液 (BGS) 是含有 100 mM SDS 和 30% (v/v) 甲醇的 50 mM 磷酸 (PA)。当在 50 毫巴下注射 100 秒时,在较短的分析时间内实现了溶解在 90 mM CD 和 100 mM PA 中的分析物的最佳分离和浓缩性能。与传统的扫描-MEKC和MEKC方法相比,使用该方法显着提高了富集效率。本方法的一系列验证研究是在最佳条件下进行的。获得了良好的线性,相关系数范围为 0.994-0.999,检测限范围为 13 到 40 ng/mL。与传统注射(注射时间 3 秒)相比,灵敏度增强因子 (SEF) 在 28.5-46.8 的范围内。因此,所提出的方法成功地应用于实际样品中中性分析物的分离和浓缩。8 与传统注射相比(注射时间3 s)。因此,所提出的方法成功地应用于实际样品中中性分析物的分离和浓缩。8 与传统注射相比(注射时间3 s)。因此,所提出的方法成功地应用于实际样品中中性分析物的分离和浓缩。
更新日期:2020-04-01
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