The wounds of diabetic patients are difficult to heal, which could lead to a limb amputation or even death. The experiment aims to develop a new type of nanoparticles that could accelerate wound healing. Epigallocatechin gallate, ascorbic acid, gelatin and chitosan nanoparticles (EV NPS) were prepared by ion cross-linking method, and their properties were studied. The optimal formula ratio of EV NPS is Vc:EGCG:Gel:CS = 0.2:3:1:1. Transmission electron microscope (TEM) images show that it is a roughly uniform spherical nanoparticle with a diameter of 200 nm. ICR mice were intraperitoneally injected with streptozocin (STZ) to establish diabetic mice. Full-thickness excisional wounds were established on the back of mice. The results showed that EV NPS can promote wound healing in diabetic mice, and the mechanism may be through increasing collagen accumulation, promoting angiogenesis and reducing the infiltration of inflammatory cells. EV NPS may have potential application values for wound healing in diabetic mice.

中文翻译：

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表没食子儿茶素没食子酸酯，抗坏血酸，明胶，壳聚糖纳米粒的制备和表征及其对糖尿病小鼠伤口愈合的有益作用。

糖尿病患者的伤口难以愈合，可能导致肢体截肢甚至死亡。该实验旨在开发一种新型的可加速伤口愈合的纳米颗粒。采用离子交联法制备了表没食子儿茶素没食子酸酯，抗坏血酸，明胶和壳聚糖纳米粒子（EV NPS），并对其性能进行了研究。EV NPS的最佳公式比率为Vc：EGCG：Gel：CS = 0.2：3：1：1。透射电子显微镜（TEM）图像显示它是直径200 nm的大致均匀的球形纳米颗粒。给ICR小鼠腹膜内注射链脲佐菌素（STZ）以建立糖尿病小鼠。在小鼠的背部建立了全层切除伤口。结果表明，EV NPS可以促进糖尿病小鼠的伤口愈合，其机制可能是通过增加胶原蛋白的积累，促进血管生成和减少炎症细胞的浸润。EV NPS可能对糖尿病小鼠的伤口愈合具有潜在的应用价值。