To preserve bioactivity and achieve colon targeted release of phycocyanin (PC), the polysaccharides-based electrospun fiber mat (EFM) containing PC and prebiotics was prepared and characterized. In vitro release tests confirmed the colon targeting behavior of PC, in particular, faster release of PC was achieved due to the addition of prebiotics. Ritger–Peppas model confirmed that the release of PC in simulated colon fluids follows a mechanism of anomalous transport (non-Fickian). CCK-8 results showed that the combination of PC and prebiotics exerted a significant anti-proliferative effect on HCT116 cells with an IC50 values of 24.17, 19.43 and 12.82 mg/mL after 24, 48, and 72 h, respectively. Furthermore, the cell cycle and apoptosis analysis revealed that the inhibition activity on HCT116 cells was caused by arresting cell cycle at G0/G1 phase that is relevant to the inhibition of cyclin D1 and CDK4 and the up-regulation of p21 expression, and inducing cell apoptosis by mediating the mitochondrial pathway as well, in which the decrease of Bcl-2/Bax, activation of caspase 3 and release of cytochrome c were included. This study suggests that the PC-loaded EFM with GOS holds a great potential as an effective formulation for colon cancer prevention.

为了保留生物活性并实现藻蓝蛋白（PC）的结肠靶向释放，制备并表征了包含PC和益生元的多糖基电纺纤维垫（EFM）。体外释放测试证实了PC的结肠靶向行为，特别是由于添加了益生元，因此PC释放更快。Ritger–Peppas模型证实模拟结肠液中PC的释放遵循异常运输（非Fickian）机制。CCK-8结果表明，PC和益生元的组合对HCT116细胞具有显着的抗增殖作用，在24、48和72小时后的IC50值分别为24.17、19.43和12.82 mg / mL。此外，c被包括在内。这项研究表明，装有PC的GOS EFM具有作为预防结肠癌的有效配方的巨大潜力。