The present study aimed to explore the effect of an anti-inflammatory RG-II type polysaccharide (KMPS) purified from Aconitum coreanum (Le'vl.) on glucose metabolism in high-fat diet-induced obese (DIO) mice. Treatment with KMPS for 4 weeks significantly reduced the fasting blood glucose, increased the sensitivity to insulin and improved glucose tolerance. Concurrently, KMPS supplementation also markedly inhibited inflammatory cytokine expression in serum and insulin target tissues and decreased the proportion of M1-type macrophages in adipose tissue, which was considered as the potential hypoglycaemic mechanism. In mechanism study, it was found that KMPS reduced the serine phosphorylation of IRS-1 by inhibiting the activation of the NF-κB pathway, thereby restoring the utilization of glucose by the PI3K/AKT pathway. These results suggested that KMPS may be a potential component for targeting inflammation in the treatment of type 2 diabetes.

本研究旨在探讨从乌头中提取的抗炎RG-II型多糖（KMPS）的作用（Lev。）对高脂饮食诱导的肥胖（DIO）小鼠葡萄糖代谢的影响。用KMPS治疗4周可显着降低空腹血糖，增加对胰岛素的敏感性并改善葡萄糖耐量。同时，补充KMPS还可以显着抑制血清和胰岛素靶组织中炎性细胞因子的表达，并降低脂肪组织中M1型巨噬细胞的比例，这被认为是潜在的降血糖机制。在机理研究中，发现KMPS通过抑制NF-κB途径的活化来降低IRS-1的丝氨酸磷酸化，从而恢复PI3K / AKT途径对葡萄糖的利用。这些结果表明，KMPS可能是治疗2型糖尿病时靶向炎症的潜在成分。