Tea tree oil (TTO) inhibits the spore germination and mycelial growth of Botrytis cinerea, and induces mitochondrial dysfunction of B. cinerea. To further determine the effects of TTO on mitochondria in B. cinerea, label-free quantitative proteomics analysis was performed. A total of 85 differentially expression proteins (DEPs) were identified; Among them 51 were more abundant in TTO-treated samples, and 34 were less abundant. DEPs were then annotated and classified into 34 functional groups based on Gene Ontology analysis. Subsequent Kyoto Encyclopedia of Genes and Genomes analysis linked identified DEPs to 83 different pathways. This study suggests that TTO inhibits the tricarboxylic acid cycle, pyruvate metabolism, amino acid metabolism, and membrane-related pathways in mitochondria, and also promotes sphingolipid metabolism, which may accelerate cell death in B. cinerea.

中文翻译：

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定量蛋白质组学揭示茶树油影响灰葡萄孢线粒体功能

茶树油 (TTO) 抑制灰葡萄孢的孢子萌发和菌丝生长，并诱导灰葡萄孢的线粒体功能障碍。为了进一步确定 TTO 对 B. cinerea 线粒体的影响，进行了无标记定量蛋白质组学分析。共鉴定了 85 个差异表达蛋白 (DEP)；其中 51 种在 TTO 处理的样品中含量较高，34 种含量较低。然后基于基因本体分析对 DEP 进行注释并分类为 34 个功能组。随后的京都基因和基因组百科全书分析将确定的 DEP 与 83 种不同的途径联系起来。这项研究表明，TTO 抑制线粒体中的三羧酸循环、丙酮酸代谢、氨基酸代谢和膜相关通路，还促进鞘脂代谢，