To identify the effect of hOGG1 methylation, Ser326Cys polymorphism and their interactions on the risk of coal-borne arsenicosis, 113 coal-borne arsenicosis subjects and 55 reference subjects were recruited. Urinary arsenic contents were analyzed with ICP-MS. hOGG1 methylation and Ser326Cys polymorphism was measured by mehtylation-specific PCR and restriction fragment length polymorphism PCR in PBLCs, respectively. The results showed that the prevalence of methylated hOGG1 and variation genotype (326 ^Ser/Cys & 326 ^Cys/Cys) were increased with raised levels of urinary arsenic in arsenicosis subjects. Increased prevalence of methylated hOGG1 and variation genotype were associated with raised risk of arsenicosis. Moreover, the results revealed that variant genotype might increase the susceptibility to hOGG1 methylation. The interactions of methylated hOGG1 and variation genotype were also found to contribute to increased risk of arsenicosis. Taken together, hOGG1 hypermethylation, hOGG1 variants and their interactions might be potential biomarkers for evaluating risk of coal-borne arsenicosis.

为了确定hOGG1甲基化，Ser326Cys多态性及其相互作用对煤媒砷中毒风险的影响，招募了113名煤媒砷中毒受试者和55名参考受试者。ICP-MS分析尿中的砷含量。hOGG1甲基化和Ser326Cys多态性分别通过PBLC中的甲基化特异性PCR和限制性片段长度多态性PCR测量。结果表明，随着砷中毒水平的升高，hOGG1甲基化的患病率和变异基因型（326 ^{Ser / Cys}和326 ^{Cys / Cys}）随着尿砷水平的升高而增加。甲基化hOGG1的患病率增加基因型和变异与砷中毒风险增加有关。此外，结果表明，变异基因型可能会增加对hOGG1甲基化的敏感性。还发现甲基化的hOGG1与变异基因型之间的相互作用会导致砷中毒的风险增加。两者合计，hOGG1甲基化，hOGG1变体及其相互作用可能是评估煤传播砷中毒风险的潜在生物标志物。