In this study, the diversity and the phylogenetic relationships of bacteria isolated from root nodules of Chamaecytisus ruthenicus growing in Poland were investigated using ERIC-PCR fingerprinting and by multilocus sequence analysis (MLSA). Two major clusters comprising 13 and 3 isolates were detected which 16S rRNA gene sequencing identified as Bradyrhizobium and Phyllobacterium. The results of phylogenetic analysis of individual and concatenated atpD, gyrB and recA gene sequences showed that the studied strains may represent novel species in the genera Bradyrhizobium and Phyllobacterium. In the phylogenetic tree based on the atpD-gyrB-recA concatemers, Bradyrhizobium isolates were split into two groups closely related to Bradyrhizobium algeriense STM89^T and Bradyrhizobium valentinum LmjM3^T. The genus Phyllobacterium isolates formed a separate cluster close to Phyllobacterium ifriqiyense LMG27887^T in the atpD-gyrB-recA phylogram. Analysis of symbiotic gene sequences (nodC, nodZ, nifD, and nifH) showed that the Bradyrhizobium isolates were most closely related to Bradyrhizobium algeriense STM89^T, Bradyrhizobium valentinum LmjM3^T and Bradyrhizobium retamae Ro19^T belonging to symbiovar retamae. This is the first report on the occurrence of members of symbiovar retamae from outside the Mediterranean region. No symbiosis related genes were amplified from Phyllobacterium strains, which were also unable to induce nodules on C. ruthenicus roots. Based on these findings Phyllobacterium isolates can be regarded as endophytic bacteria inhabitating root nodules of C. ruthenicus.

在这项研究中，分集和从根瘤中分离的细菌的系统发育关系Chamaecytisus ruthenicus在波兰生长用ERIC-PCR指纹和通过多位点序列分析（MLSA）研究。进行检测，其包括13个3株两个主要簇其16S rRNA基因测序鉴定为慢生根瘤菌和Phyllobacterium。个人和级联的系统发育分析的结果ATPD，gyrB基因和recA的基因序列表明，所研究的菌株可以表示属新种根瘤菌和Phyllobacterium。在基于atpD-gyrB-recA串联体，将根瘤菌分离株分为两个群，分别与algeriense STM89 ^T和valentinum LmjM3 ^T密切相关。属Phyllobacterium菌株形成一个单独的集群接近Phyllobacterium ifriqiyense LMG27887 ^Ť在ATPD-的gyrB-recA启动系统发生。共生的基因序列（分析NODC，nodZ，nifD和nifH），显示根瘤菌菌株最密切相关慢生algeriense STM89 ^Ť，Bradyrhizobium属valentinum LmjM3 ^Ť和慢生retamae Ro19 ^Ť属于symbiovar retamae。这是关于地中海地区以外共生菌共生成员发生的第一份报告。没有共生相关基因从扩增Phyllobacterium株，这也不能诱导对结节C. ruthenicus根。基于这些发现，Phyllobacterium分离物可以被认为是内生细菌inhabitating根瘤C. ruthenicus。