Fifteen enterobacterial strains were isolated from fresh produce. The 16S rRNA gene sequences indicated that these belong to Serratia, with twelve strains showing 99.57%–99.93% and three strains showing 99.86–100% 16S rRNA gene sequence similarity with Serratia marcescens and Serratia nematodiphila as nearest neighbors, respectively. Further comparative multi locus sequence analyses, as well as phylogenomic comparisons, revealed that 6 of the 15 strains were well-separated from their nearest neighbors and formed two clearly distinct taxa. Strains S2, S9, S10 and S15^T were urease-positive, while strains S3^T and S13 were urease-negative. Average nucleotide identity and digital DNA–DNA hybridization comparisons of representative strains S3^T and S15^T with type strains of S. marcescens, S. nematodiphila and S. ureilytica indicated that these shared less than 96% and 70% homology, respectively. Major fatty acids of strains S3^T and S15^T included C16:0, C16:1 ω7c/C16:1 ω6c, C17:0 Cyclo and C18:1 ω6c /C18:1 ω7c. The mol% G + C of genomic DNA of strain S15^T was 59.49% and of strain S3^T was 59.04. These results support the description of two novel species, Serratia nevei and Serratia bockelmannii, with strains S15^T (=LMG 31536^T =DSM 110085^T) and S3^T (=LMG 31535^T =DSM 110152^T) as type strains, respectively. Although Serratia marcescens subsp. sakuensis was previously described to form spores, spores could not be determined in this study. As spore formation was the only differential characteristic of this subspecies, S. marcescens subsp. sakuensis is a later heterotypic synonym of Serratia marcescens.

从新鲜产品中分离出十五种肠杆菌菌株。16S rRNA基因序列表明它们属于粘质沙雷氏菌，其中十二个菌株显示了99.57％–99.93％，三个菌株显示了99.86–100％的16S rRNA基因序列相似性，分别与粘质沙雷氏菌和线虫沙雷氏菌为最近的邻居。进一步的比较多基因座序列分析以及系统生物学比较显示，这15个菌株中有6个与它们的近邻完全分开，并形成了两个明显不同的类群。菌株S2，S9，S10和S15 ^T为脲酶阳性，而菌株S3 ^T为和S13是脲酶阴性的。平均核苷酸同一性和代表菌株S3的数字DNA-DNA杂交的比较^Ť和S15 ^Ť用的型菌株粘质沙雷氏菌，S. nematodiphila和S. ureilytica表明这些分别共享小于96％和70％的同源性。S3 ^T和S15 ^T菌株的主要脂肪酸包括C16：0，C16：1ω7c/ C16：1ω6c，C17：0 Cyclo和C18：1ω6c/ C18：1ω7c。菌株S15 ^T的基因组DNA的mol％G + C为59.49％，菌株S3 ^T的为59.04。这些结果支持描述了两个新物种，沙雷氏菌和沙雷氏菌。沙雷氏菌，分别具有菌株S15 ^T（= LMG 31536 ^T = DSM 110085 ^T）和S3 ^T（= LMG 31535 ^T = DSM 110152 ^T）作为类型菌株。尽管粘质沙雷氏菌（Serratia marcescens）亚种。之前曾描述过sakuensis会形成孢子，但这项研究无法确定孢子。由于孢子的形成是该亚种的唯一差异特征，因此，S。marcescens亚种。sakuensis是粘质沙雷氏菌（Serratia marcescens）的后来的异型同义词。