Hepcidin, a multifunctional hormone oligopeptide, not only exhibits a regulatory role in iron metabolism, but also participates in the regulation of teleostean immunity. In this study, ORF sequence of WR-hepcidin was 258 bp and encoded 85 amino acid residues. Tissue-specific analysis revealed that the highest expression of WR-hepcidin was observed in liver. Aeromonas hydrophila challenge can sharply increased WR-hepcidin mRNA expression in liver, trunk kidney and spleen. The purified WR-hepcidin fusion peptide can directly bind to A. hydrophila and Streptococcus agalactiae, reduce the relative bacterial activity, limit bacterial growth and attenuate their dissemination to tissues in vivo. In addition, the treatment of WR-hepcidin fusion protein can diminish the production of pro-inflammatory cytokines. These results indicated that WR-hepcidin can play a negative regulatory role in bacteria-stimulated pro-inflammatory cytokines production and MyD88-IRAK4 activation.

中文翻译：

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一种新型铁调素同源物在二倍体杂种鱼中的抗菌和免疫调节活性（Car鱼♀鱼assi红色变种♂）。

Hepcidin是一种多功能激素寡肽，不仅在铁代谢中发挥调节作用，而且还参与硬骨鱼免疫力的调节。在这项研究中，WR-铁调素的ORF序列为258 bp，编码85个氨基酸残基。组织特异性分析显示，在肝中观察到WR-hepcidin的最高表达。嗜水气单胞菌攻击可急剧增加肝，躯干肾脏和脾脏中WR-hepcidin mRNA表达。纯化的WR-hepcidin融合肽可以直接结合亲水性链球菌和无乳链球菌，降低相对细菌活性，限制细菌的生长并减弱它们在体内向组织的传播。另外，WR-铁调素融合蛋白的治疗可以减少促炎细胞因子的产生。