In this paper, we report the real-time, ultrasensitive, reproducible detection of volatile odorant molecules emitted by human based on free-standing silicon nanowire (SiNW) array functionalized with Anopheles gambiae odorant binding protein (AgOBP). Highly responsive SiNW array was fabricated with a novel low-cost top-down fabrication approach. The free-standing and triangular cross sectional structure allowed efficient sensing of AgOBP conformational change induced by odorant binding. The resulting bioelectronic nose (B-nose) possessed high sensitivity down to several ppb, and outstanding size and functional group selectivity. The device showed reproducible and long-lasting performances. We also demonstrated the multiplex detection of various odorants with a sensor array functionalized with three different AgOBPs. The AgOBP-SiNW B-nose reported herein provides a powerful and effective platform for future development of multiplexed human body odor sensor arrays.

在本文中，我们报告了基于冈比亚按蚊气味结合蛋白（Ag OBP）功能化的独立式硅纳米线（SiNW）阵列，实时，超灵敏，可重现地检​​测人发出的挥发性气味分子。高响应性SiNW阵列是通过一种新颖的低成本自上而下的制造方法制造的。独立的三角形横截面结构可有效检测Ag气味结合引起的OBP构象变化。所得的生物电子鼻（B鼻）具有低至几ppb的高灵敏度，并具有出色的尺寸和官能团选择性。该设备显示出可再现且持久的性能。我们还演示了使用三种不同的Ag OBP功能化的传感器阵列对多种气味剂进行的多重检测。本文报道的Ag OBP-SiNW B-鼻孔提供了强大且有效的平台，用于未来开发的多种人体气味传感器阵列。