当前位置: X-MOL 学术Mol. Biol. Evol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Evolution of Transcriptional Repressors Impacts Caenorhabditis Vulval Development.
Molecular Biology and Evolution ( IF 10.7 ) Pub Date : 2020-05-01 , DOI: 10.1093/molbev/msaa009
Helen M Chamberlin 1 , Ish M Jain 2 , Marcos Corchado-Sonera 1 , Leanne H Kelley 1 , Devika Sharanya 2 , Abdulrahman Jama 1 , Romy Pabla 2 , Adriana T Dawes 1, 3 , Bhagwati P Gupta 2
Affiliation  

Comparative genomic sequence analysis has found that the genes for many chromatin-associated proteins are poorly conserved, but the biological consequences of these sequence changes are not understood. Here, we show that four genes identified for an Inappropriate Vulval cell Proliferation (ivp) phenotype in the nematode Caenorhabditis briggsae exhibit distinct functions and genetic interactions when compared with their orthologs in C. elegans. Specifically, we show that the four C. briggsae ivp genes encode the noncanonical histone HTZ-1/H2A.z and three nematode-specific proteins predicted to function in the nucleus. The mutants exhibit ectopic vulval precursor cell proliferation (the multivulva [Muv] phenotype) due to inappropriate expression of the lin-3/EGF gene, and RNAseq analysis suggests a broad role for these ivp genes in transcriptional repression. Importantly, although the C. briggsae phenotypes have parallels with those seen in the C. elegans synMuv system, except for the highly conserved HTZ-1/H2A.z, comparable mutations in C. elegans ivp orthologs do not exhibit synMuv gene interactions or phenotypes. These results demonstrate the evolutionary changes that can underlie conserved biological outputs and argue that proteins critical to repress inappropriate expression from the genome participate in a rapidly evolving functional landscape.

中文翻译:

转录阻遏物的演变会影响秀丽隐杆线虫的外阴发育。

比较基因组序列分析发现,许多染色质相关蛋白的基因保守性很差,但是这些序列变化的生物学结果尚不清楚。在这里,我们显示了在线虫Caenorhabditis briggsae中为不适当的外阴细胞增殖(ivp)表型鉴定的四个基因,与在秀丽隐杆线虫的直系同源物相比,表现出不同的功能和遗传相互作用。具体来说,我们表明,四个炭疽病ivp基因编码非规范的组蛋白HTZ-1 / H2A.z和三个预测在核中起作用的线虫特异性蛋白。由于lin-3 / EGF基因的不适当表达,这些突变体表现出异位外阴前体细胞增殖(多外阴[Muv]表型),RNAseq分析表明这些ivp基因在转录抑制中具有广泛的作用。重要的是,尽管秀丽隐杆线虫的表型与秀丽隐杆线虫synMuv系统中的相似,除了高度保守的HTZ-1 / H2A.z外,秀丽隐杆线虫ivp直系同源基因的可比突变没有表现出synMuv基因相互作用或表型。 。这些结果证明了进化变化可能是保守的生物学输出的基础,并认为抑制基因组中不适当表达的关键蛋白参与了快速发展的功能领域。线虫ivp直向同源物不显示synMuv基因相互作用或表型。这些结果证明了进化变化可能是保守的生物学输出的基础,并认为抑制基因组中不适当表达的关键蛋白参与了快速发展的功能领域。线虫ivp直向同源物不显示synMuv基因相互作用或表型。这些结果证明了进化变化可能是保守的生物学输出的基础,并认为抑制基因组中不适当表达的关键蛋白参与了快速发展的功能领域。
更新日期:2020-01-21
down
wechat
bug