Background

This study investigated depot-specific mRNA expression of uncoupling protein 1 (UCP1) in human white adipose tissue (WAT) and its association with obesity-related markers.

Methods

We recruited 39 normal-weight, 41 nondiabetic obese, and 22 diabetic obese women. We measured UCP1 mRNA expression in abdominal visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT), and investigated the associations between UCP1 mRNA expression in VAT and SAT, and obesity-related markers including mRNA expression of leptin, adiponectin, CCAAT-enhancer-binding protein homologous protein (CHOP), and positive regulatory domain-containing protein 16 (PRDM16). We also evaluated UCP1 mRNA expression in differentiated human white adipocytes after treatment with various stressors and metabolic improvement agents in vitro.

Results

UCP1 mRNA in VAT was significantly higher than in SAT in all groups. UCP1 mRNA in SAT was negatively correlated with BMI, total abdominal fat area, visceral fat area, blood pressure, fasting glucose, insulin, HOMA-IR score, triglyceride, hsCRP, fasting leptin levels, and adipocyte size. UCP1 mRNA in SAT was positively correlated with fasting adiponectin levels. UCP1 mRNA in VAT was negatively correlated with visceral-to-subcutaneous fat ratio (VSR), fasting glucose, and triglyceride levels. In SAT, UCP1 mRNA was negatively correlated with mRNA expression of leptin and CHOP, and positively correlated with mRNA expression of adiponectin. The expression of PRDM16 was positively correlated with UCP1 mRNA in both VAT and SAT. UCP1 mRNA expression in differentiated human white adipocytes was significantly reduced after incubation with thapsigargin, tunicamycin, homocysteine, TNF-α, or IL-β, and significantly increased after incubation with exendin 4, dapagliflozin, and telmisartan.

Conclusions

This study demonstrated depot-specific mRNA expression of UCP1 and its association with obesity-related markers in human WAT. UCP1 mRNA in human white adipocytes was suppressed by inflammatory agents and enhanced by metabolic improvement agents. UCP1 in human WAT might participate in the pathogenesis of obesity-related metabolic diseases.

中文翻译：

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人白色脂肪组织中的仓库特异性 UCP1 表达及其与肥胖相关标志物的关联

背景

本研究调查了人白色脂肪组织 (WAT) 中解偶联蛋白 1 ( UCP1 ) 的仓库特异性 mRNA 表达及其与肥胖相关标志物的关联。

方法

我们招募了 39 名正常体重、41 名非糖尿病肥胖和 22 名糖尿病肥胖女性。我们测量UCP1基因表达的腹部内脏脂肪组织（VAT）和皮下脂肪组织（SAT），并研究之间的关联UCP1基因表达的VAT和SAT，和肥胖相关的标记物，包括瘦素，脂联素，CCAAT增强子的mRNA的表达结合蛋白同源蛋白 ( CHOP ) 和含有正调节结构域的蛋白 16 ( PRDM16 )。我们还在体外用各种应激源和代谢改善剂处理后评估了分化的人白色脂肪细胞中UCP1 mRNA 的表达。

结果

在所有组中，VAT 中的UCP1 mRNA 显着高于 SAT。SAT中的UCP1 mRNA与BMI、腹部总脂肪面积、内脏脂肪面积、血压、空腹血糖、胰岛素、HOMA-IR评分、甘油三酯、hsCRP、空腹瘦素水平和脂肪细胞大小呈负相关。SAT中的UCP1 mRNA与空腹脂联素水平呈正相关。VAT中的UCP1 mRNA与内脏皮下脂肪比（VSR）、空腹血糖和甘油三酯水平呈负相关。SAT中UCP1 mRNA与leptin和CHOP mRNA表达呈负相关，与脂联素mRNA表达呈正相关。PRDM16的表达在 VAT 和 SAT 中与UCP1 mRNA呈正相关。与毒胡萝卜素、衣霉素、同型半胱氨酸、TNF-α 或 IL-β 孵育后，分化的人白色脂肪细胞中UCP1 mRNA 表达显着降低，而与毒蜥外泌肽 4、达格列净和替米沙坦孵育后显着增加。

结论

该研究证明了UCP1的仓库特异性 mRNA 表达及其与人类 WAT 中肥胖相关标志物的关联。人白色脂肪细胞中的UCP1 mRNA 被炎症剂抑制并被代谢改善剂增强。人类WAT中的UCP1可能参与肥胖相关代谢疾病的发病机制。