BACKGROUND We previously proposed a technique for quantitative measurement of rest and stress absolute myocardial blood flow (MBF) using a 2-injection single-scan imaging session. Recently, we validated the method in a pig model for the long-lived radiotracer 18F-Flurpiridaz with adenosine as a pharmacological stressor. The aim of the present work is to validate our technique for 13NH3. METHODS Nine studies were performed in 6 pigs; 5 studies were done in the native state and 4 after infarction of the left anterior descending artery. Each study consisted of 3 dynamic scans: a 2-injection rest-rest single-scan acquisition (scan A), a 2-injection rest/stress single-scan acquisition (scan B), and a conventional 1-injection stress acquisition (scan C). Variable doses of adenosine combined with dobutamine were administered to induce a wide range of MBF. The 2-injection single-scan measurements were fitted with our nonstationary kinetic model (MGH2). In 4 studies, 13NH3 injections were paired with microsphere injections. MBF estimates obtained with our method were compared with those obtained with the standard method and with microspheres. We used a model-based method to generate separate rest and stress perfusion images. RESULTS In the absence of stress (scan A), the MBF values estimated by MGH2 were nearly the same for the 2-radiotracer injections (mean difference: 0.067±0.070 mL·min-1·cc-1, limits of agreement: [-0.070 to 0.204] mL·min-1·cc-1), showing good repeatability. Bland-Altman analyses demonstrated very good agreement with the conventional method for both rest (mean difference: -0.034±0.035 mL·min-1·cc-1, limits of agreement: [-0.103 to 0.035] mL·min-1·cc-1) and stress (mean difference: 0.057±0.361 mL·min-1·cc-1, limits of agreement: [-0.651 to 0.765] mL·min-1·cc-1) MBF measurements. Positron emission tomography and microsphere MBF measurements correlated closely. Very good quality perfusion images were obtained. CONCLUSIONS This study provides in vivo validation of our single-scan rest-stress method for 13NH3 measurements. The 13NH3 rest/stress myocardial perfusion imaging procedure can be compressed into a single positron emission tomography scan session lasting less than 15 minutes.

中文翻译：

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用于13N氨正电子发射断层扫描心脏灌注研究的单扫描静息/压力成像技术的临床前验证。

背景技术我们先前提出了一种使用两次注射单次扫描成像会话来定量测量静止和压力绝对心肌血流量（MBF）的技术。最近，我们在以腺苷为药理应激源的长寿命放射性示踪剂18F-Flurpiridaz的猪模型中验证了该方法。当前工作的目的是验证我们的13NH3技术。方法对6头猪进行了9项研究。在原始状态下进行了5项研究，在左前降支梗死后进行了4项研究。每项研究均包括3次动态扫描：一次2次静息-静止单次扫描采集（扫描A），一次2次静息/应激单次扫描采集（扫描B次）和常规的一次一次注入应力采集（一次扫描） C）。给予不同剂量的腺苷联合多巴酚丁胺以诱导大范围的MBF。两次注射单次扫描测量值符合我们的非平稳动力学模型（MGH2）。在4项研究中，将13NH3注射液与微球注射液配对使用。我们的方法获得的MBF估计值与标准方法和微球获得的MBF估计值进行了比较。我们使用基于模型的方法来生成单独的休息和压力灌注图像。结果在没有压力的情况下（扫描A），由MGH2估算的2次放射性示踪剂注射的MBF值几乎相同（均差：0.067±0.070 mL·min-1·cc-1，一致极限：[- 0.070至0.204] mL·min-1·cc-1），显示出良好的重复性。Bland-Altman分析表明，两种方法均与常规方法非常吻合（均值差：-0。034±0.035 mL·min-1·cc-1，一致极限：[-0.103至0.035] mL·min-1·cc-1）和应力（均值：0.057±0.361 mL·min-1·cc-） 1，一致性极限：[-0.651至0.765] mL·min-1·cc-1）MBF测量。正电子发射断层扫描与微球MBF测量值密切相关。获得了非常高质量的灌注图像。结论本研究为13NH3测量的单扫描静应力方法提供了体内验证。可以将13NH3静息/应激心肌灌注成像过程压缩为单个正电子发射断层扫描扫描过程，持续时间少于15分钟。正电子发射断层扫描与微球MBF测量值密切相关。获得了非常高质量的灌注图像。结论本研究为13NH3测量的单扫描静应力方法提供了体内验证。可以将13NH3静息/应激心肌灌注成像过程压缩为单个正电子发射断层扫描扫描过程，持续时间少于15分钟。正电子发射断层扫描与微球MBF测量值密切相关。获得了非常高质量的灌注图像。结论本研究为13NH3测量的单扫描静应力方法提供了体内验证。可以将13NH3静息/应激心肌灌注成像过程压缩为单个正电子发射断层扫描扫描过程，持续时间少于15分钟。