Group 2 innate lymphoid cells (ILC2s) are enriched at mucosal sites, including the lung, and play a central role in type 2 immunity and maintaining tissue homeostasis. As a result, since their discovery in 2010, research into ILC2s has increased markedly. Numerous strategies have been used to define ILC2s by flow cytometry, often utilizing different combinations of surface markers despite their expression being variable and context-dependent. In this study, we sought to generate a comprehensive characterization of pulmonary ILC2s, identifying stable and context specific markers from different pulmonary compartments following different airway exposures in different strains of mice. Our analysis revealed that pulmonary ILC2 surface marker phenotype is heterogeneous and is influenced by mouse strain, pulmonary location, in vivo treatment/exposure and ex vivo stimulation. Therefore, we propose that a lineage negative cell expressing CD45 and Gata3 defines an ILC2, and subsequent surface marker expression should be used to describe their phenotype in context-specific scenarios.

中文翻译：

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肺组2先天淋巴样细胞表型是特定于上下文的：确定应变，位置和刺激的影响。

第2组先天性淋巴样细胞（ILC2）在包括肺在内的粘膜部位富集，并在2型免疫和维持组织动态平衡中发挥重要作用。结果，自2010年被发现以来，对ILC2的研究显着增加。已经通过流式细胞术使用了许多策略来定义ILC2，尽管它们的表达是可变的并且取决于上下文，但经常利用表面标记的不同组合。在这项研究中，我们寻求生成肺ILC2的全面表征，在不同品系的小鼠中，在不同的气道暴露后，从不同的肺室中鉴定出稳定的和特定情境的标志物。我们的分析表明，肺ILC2表面标记的表型是异质的，并且受小鼠品系，肺位置，体内治疗/接触和离体刺激。因此，我们建议表达CD45和Gata3的谱系阴性细胞定义一个ILC2，随后的表面标记表达应用于描述在特定情况下的表型。