Abstract

An existing procedure for the separation and purification of glucose in sake for compound-specific carbon stable isotope analysis was examined with a view to reducing the sample amount required and process complexity, with a focus on the freeze-drying, solid-phase extraction (SPE), and high-performance liquid chromatography (HPLC) steps. In the revised procedure, the carbon stable isotopic composition, as indicated by δ¹³C values, of glucose was not significantly affected by glucose concentration in the range 0.25–1.00 mg mL⁻¹, solvent (water or 80% aqueous acetonitrile), or HPLC procedure. Glucose freeze-dried after separation and purification by SPE and HPLC exhibited procedure-induced carbon isotopic discrimination of ≤ 0.1‰. The isolation procedure achieved the same accuracy in glucose δ¹³C values as that achieved with traditional ion-exchange procedures while requiring a sample volume (1 mL) only 4% that of the usual volume. The improved method enables cost-effective, labor-efficient, small-scale, compound-specific carbon stable isotope analyses of glucose in sake.

中文翻译：

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酒中葡萄糖的分离纯化用于碳稳定同位素分析

摘要

为了减少所需的样品量和工艺复杂性，研究了用于化合物特异性碳稳定同位素分析的现有葡萄糖分离和纯化方法，重点是冷冻干燥固相萃取（SPE）。 ）和高效液相色谱（HPLC）步骤。在修改后的过程中，碳稳定同位素组成，通过所指示的δ ¹³ C值，葡萄糖不显著在范围0.25-1.00毫克毫升受葡萄糖浓度^-1，溶剂（水或80％乙腈水溶液）或HPLC程序。经SPE和HPLC分离纯化后冷冻干燥的葡萄糖表现出程序诱导的碳同位素分辨力≤0.1‰。分离步骤实现了葡萄糖相同的精度δ ^13个C值作为与传统的离子交换程序来实现，同时需要的样品体积（1mL）中只有4％的是，通常的体积。改进的方法可以实现对葡萄糖的经济高效，省力，小规模，化合物特定的碳稳定同位素分析。