Designing synthetic pathways for efficient CO₂ fixation and conversion is essential for sustainable chemical production. Here we have designed a synthetic acetate‐acetyl‐CoA/malonyl‐CoA (AAM) bypass to overcome an enzymatic activity of pyruvate dehydrogenase complex. This synthetic pathway utilizes acetate assimilation and carbon rearrangements using a methyl malonyl‐CoA carboxyltransferase. We demonstrated direct conversion of CO₂ into acetyl‐CoA‐derived acetone as an example in photosynthetic Synechococcus elongatus PCC 7942 by increasing the acetyl‐CoA pools. The engineered cyanobacterial strain with the AAM‐bypass produced 0.41 g/L of acetone at 0.71 m/day of molar productivity. This work clearly shows that the synthetic pyruvate dehydrogenase bypass (AAM‐bypass) is a key factor for the high‐level production of an acetyl‐CoA‐derived chemical in photosynthetic organisms.

中文翻译：

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合成丙酮酸脱氢酶旁路的代谢重新连接，用于在蓝细菌中生产丙酮。

设计有效的CO ₂固定和转化的合成途径对于可持续化学生产至关重要。在这里，我们设计了一种合成的乙酸-乙酰-CoA /丙二酰-CoA（AAM）旁路技术，以克服丙酮酸脱氢酶复合物的酶促活性。该合成途径利用甲基丙二酰-CoA羧基转移酶利用乙酸酯同化作用和碳重排。我们证明了将CO ₂直接转化为乙酰-CoA衍生的丙酮为例，在光合作用伸长球突触球菌中通过增加乙酰辅酶A库增加PCC 7942。带有AAM旁路的工程蓝细菌菌株以0.71 m / day的摩尔生产率产生0.41 g / L的丙酮。这项工作清楚地表明，合成的丙酮酸脱氢酶旁路（AAM旁路）是在光合生物中高水平生产乙酰辅酶A衍生化学品的关键因素。