Electrochemical sensing is an effective, low-cost technology for cancer detection. In this study, mesoporous TiO2 was prepared via biomimetic synthesis based on yeast cell templates, and used to prepare a modified electrode for the sensitive detection of pancreatic cancer miR-1290. The structure and the morphology of the TiO2 were characterized by X-ray diffraction (XRD), N2 adsorption-desorption isotherm (NADI), Atomic force microscopy (AFM), and electron probe microanalysis (EPMA). As a sensing active material, the yeast-templated mesoporous TiO2 could detect pancreatic cancer miRNAs with single-nucleotide discrimination. The sample prepared by calcination at 400 °C showed the best electrochemical sensing activity. Moreover, compared with the blank electrode, the yeast mesoporous TiO2 sensing electrode could oxidize the pancreatic cancer microRNAs at a lower potential, which minimized the interference from oxygen evolution reaction at high potentials.

中文翻译：

---

基于酵母模板化介孔 TiO2 修饰电极的胰腺癌 miR-1290 的电化学传感

电化学传感是一种有效、低成本的癌症检测技术。在本研究中，基于酵母细胞模板通过仿生合成制备了介孔 TiO2，并用于制备修饰电极，用于灵敏检测胰腺癌 miR-1290。通过X射线衍射（XRD）、N2吸附-解吸等温线（NADI）、原子力显微镜（AFM）和电子探针微量分析（EPMA）对TiO2的结构和形貌进行了表征。作为一种传感活性材料，酵母模板化的介孔 TiO2 可以通过单核苷酸区分检测胰腺癌 miRNA。通过在 400 °C 下煅烧制备的样品显示出最好的电化学传感活性。此外，与空白电极相比，