Proteomic analysis of differentially expressed proteins in the lipopolysaccharide-stimulated hepatopancreas of the freshwater crayfish Procambarus clarkii.,Fish & Shellfish Immunology

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Proteomic analysis of differentially expressed proteins in the lipopolysaccharide-stimulated hepatopancreas of the freshwater crayfish Procambarus clarkii.
Fish & Shellfish Immunology ( IF 4.7 ) Pub Date : 2020-01-20 , DOI: 10.1016/j.fsi.2020.01.033
Ying-Yu Tang ₁ , Qiu-Ning Liu ₂ , Cheng Wang ₃ , Ting-Ting Yang ₄ , Bo-Ping Tang ₃ , Chun-Lin Zhou ₃ , Li-Shang Dai ₅

Affiliation

Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetland, Yancheng Teachers University, Yancheng, 224007, PR China; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, PR China; College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 210009, PR China.
Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetland, Yancheng Teachers University, Yancheng, 224007, PR China; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, PR China.
Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetland, Yancheng Teachers University, Yancheng, 224007, PR China.
Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetland, Yancheng Teachers University, Yancheng, 224007, PR China; College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 210009, PR China.
School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, PR China.

Procambarus clarkii is one of the most important aquatic invertebrates in China and has high commercial value. However, aquaculture has suffered great economic loss due to outbreaks of infectious diseases in P. clarkii. To identify red swamp crayfish related proteins involved in the response to bacterial infection, we analysed immune-related proteins following lipopolysaccharide (LPS) stimulation by quantitative proteomics. The proteome of the hepatopancreas of P. clarkii challenged with LPS and phosphate-buffered saline was analysed to evaluate the immune response. Based on liquid chromatography coupled with tandem mass spectrometry, 16 upregulated and 29 downregulated proteins were identified. A Gene Ontology analysis demonstrated 5 biological process, 11 cellular component, and 6 molecular function subcategories. The Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that the identified proteins were mainly involved in metabolism, phagosome, and ribosome. Real-time quantitative reverse transcription-PCR revealed that eight immune-related genes were upregulated after LPS stimulation compared to the control. Taken together, the data enhance our understanding of the immune response of crayfish to LPS.

更新日期：2020-01-21

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