The characteristic vibrational spectroscopic fingerprint of Raman reporter molecules adsorbed on noble metal nanoparticles is employed for the identification of target proteins by the corresponding surface‐enhanced Raman scattering (SERS) nanotag‐labeled antibodies. Here, we present the modular synthesis of thiolated polyenes with two to five C═C double bonds introduced via stepwise Wittig reactions. The experimental characterization of their electronic and vibrational properties is complemented by density functional theory calculations. Highly SERS‐active nanotags are generated by using the thiolated polyenes as Raman reporter molecules in Au/Au core/satellite supraparticles with multiple hot spots. The cytokines IL‐1β and IFN‐γ are detected in a duplex SERS‐based lateral flow assay on a nitrocellulose test strip by Raman microscopy. The thiolated polyenes are suitable for use in immuno‐SERS applications such as point‐of‐care testing as well as cellular and tissue imaging.

中文翻译：

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合理设计硫醇化多烯作为表面增强拉曼散射纳米标签中三官能拉曼报告分子，以在侧向流动分析中检测细胞因子。

吸附在贵金属纳米颗粒上的拉曼报告分子的特征性振动光谱指纹图谱通过相应的表面增强拉曼散射（SERS）纳米标签标记的抗体用于目标蛋白的鉴定。在这里，我们介绍了通过逐步的Wittig反应引入的具有2至5个C═C双键的硫醇化多烯的模块化合成方法。它们的电子和振动特性的实验表征得到密度泛函理论计算的补充。通过使用硫醇化多烯作为具有多个热点的Au / Au核/卫星超颗粒中的拉曼报告分子，可以产生高度SERS活性的纳米标签。通过拉曼显微镜在硝酸纤维素试纸上的基于SERS的双侧向横向测定法中检测到细胞因子IL-1β和IFN-γ。