Lignin is the second most abundant polymer after cellulose in lignocellulosic biomass. Its aromatic composition and recalcitrant nature make its valorization a major challenge for obtaining low molecular weight aromatics compounds with high value‐added from the enzymatic depolymerization of industrial lignins. The oxidation reaction of lignin polymer using laccases alone remains inefficient. Therefore, researches are focused on the use of a laccase‐mediator system (LMS) to facilitate enzymatic depolymerization. Until today, the LMS system was studied using water‐soluble lignin only (commercial lignins, modified lignins, or lignin model compounds). This work reports a study of three LMS systems to depolymerize the three major industrial lignins (organosolv lignin, Kraft lignin, and sodium lignosulfonate). We show that an enzymatic depolymerization of these lignins can be achieved by LMS using laccase from Trametes versicolor, 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) diammonium salt as mediator and a cosolvent (25% of 1,4‐dioxane) to enhance the solubilization of lignins.

中文翻译：

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在1，4-二恶烷/水中通过漆酶-介体系统对工业木质素进行酶促解聚。

木质素是仅次于纤维素的木质纤维素生物质中第二富裕的聚合物。它的芳族成分和难降解性使它的增价成为从工业木质素的酶促解聚中获得高附加值的低分子量芳族化合物的主要挑战。仅使用漆酶的木质素聚合物的氧化反应仍然无效。因此，研究集中在使用漆酶-介体系统（LMS）促进酶促解聚。直到今天，仅使用水溶性木质素（商业木质素，改性木质素或木质素模型化合物）研究LMS系统。这项工作报告了对三个LMS系统进行解聚以研究三种主要工业木质素（有机溶剂木质素，卡夫木质素和木质素磺酸钠）的研究。杂色曲霉，2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）二铵盐作为介质和助溶剂（1,4-二恶烷的25％）可增强木质素的增溶作用。