Losartan potassium (LOS) and atorvastatin (ATR) are used in combination for long-term treatment of stroke and for treatment of hypertension with high-level cholesterol. Both drugs were simultaneously determined and validated using a novel, easy, fast, and economical first-derivative synchronous fluorescence spectroscopic method. Methanol was used as the solvent for both drugs at a Δλ 80 nm and with a scanning rate of 600 nm/min. Peaks were determined as at 288.1 nm and 263.6 nm for LOS and ATR, respectively. The proposed method was validated according to International Conference on Harmonization guidelines and, subsequently, the developed method was applicable to the analysis of the two compounds in their different formulations without interference from each other. Amplitude-concentration plots were rectilinear over the concentration ranges 1.0-10.0 μg/ml and 0.5-5.0 μg/ml for LOS and ATR, respectively. Detection limits were found to be 0.096 μg/ml and 0.030 μg/ml and quantitation limits were 0.291 μg/ml and 0.093 μg/ml for LOS and ATR, respectively. The proposed method was successfully applied to the analysis of both compounds in synthetic mixtures and in laboratory-prepared tablets. These results were in accordance with the results acquired using the comparison method, high-performance liquid chromatography.

中文翻译：

---

用一阶导数同步荧光光谱法估算氯沙坦钾和阿托伐他汀的纯净形式和片剂含量。

氯沙坦钾（LOS）和阿托伐他汀（ATR）联合用于中风的长期治疗和高胆固醇血症的高血压治疗。两种药物同时使用新颖，简便，快速且经济的一阶导数同步荧光光谱法进行测定和验证。甲醇用作两种药物的溶剂，Δλ80 nm，扫描速度为600 nm / min。LOS和ATR的峰分别位于288.1 nm和263.6 nm。根据国际协调会议指南对提出的方法进行了验证，随后，所开发的方法适用于两种化合物的不同配方分析，而不会互相干扰。振幅浓度图在浓度范围1.0-10上呈直线。LOS和ATR分别为0μg/ ml和0.5-5.0μg/ ml。LOS和ATR的检出限分别为0.096μg/ ml和0.030μg/ ml，定量限分别为0.291μg/ ml和0.093μg/ ml。所提出的方法已成功地用于分析合成混合物和实验室制备的片剂中的两种化合物。这些结果与使用比较方法高效液相色谱法获得的结果一致。