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Enhanced biodegradation of n-Hexadecane in solid-phase of soil by employing immobilized Pseudomonas Aeruginosa on size-optimized coconut fibers.
Journal of Hazardous Materials ( IF 13.6 ) Pub Date : 2020-01-18 , DOI: 10.1016/j.jhazmat.2020.122134
Mahdiyeh Hajieghrari 1 , Parisa Hejazi 1
Affiliation  

In this research, biodegradation of hexadecane as a model contaminant in solid soil using both free and immobilized Pseudomonas Aeruginosa, capable of producing biosurfactant, was investigated. Coconut fibers in three mesh sizes were used as a cellulosic biocarrier for immobilization procedure. Bioremediation experiments were monitored for 60 days after incubation at 27 °C in small columns, containing contaminated solid soil, with the capability of aeration from bottom to top. The difference in the number of immobilized bacteria cells on the fibers with different particle sizes, emphasizes the importance of choosing an optimized carrier size. Enhancement in hexadecane degradation up to 50 % at the end of experiments was achieved by immobilized Pseudomonas Aeruginosa on the fibers with a mesh size between 8 and 16 compared to inoculation of free bacteria cells into the soil. Effect of mixing the pretreated fibers with soil and inoculating free cells into this mixture was also investigated compared to free cell experiments without fiber, which led to 28 % decrease in hexadecane degradation. Obtained kinetic equations for experiments confirm the impact of immobilization of bacteria on the enhancement of biodegradation rate and reduction of the half-life of the contaminant is soil.

中文翻译:

通过在尺寸优化的椰子纤维上固定化铜绿假单胞菌,可增强土壤中固相正十六烷的生物降解能力。

在这项研究中,研究了使用能够产生生物表面活性剂的游离铜绿假单胞菌和固定化铜绿假单胞菌对固体土壤中十六烷作为模型污染物的生物降解。将三种筛孔大小的椰子纤维用作固定步骤的纤维素生物载体。在27°C下在装有污染固体土壤的小柱中温育60天后,对生物修复实验进行监测,并具有从底部到顶部通气的能力。具有不同粒径的纤维上固定的细菌细胞数量的差异,强调了选择最佳载体尺寸的重要性。与将游离细菌细胞接种到土壤中相比,通过将铜绿假单胞菌固定在网眼大小为8至16的纤维上,可以在实验结束时将十六烷降解提高多达50%。与没有纤维的自由细胞实验相比,还研究了将预处理的纤维与土壤混合并在该混合物中接种游离细胞的效果,这导致十六烷降解降低了28%。获得的用于实验的动力学方程式证实了固定化细菌对提高生物降解速率和降低土壤污染物半衰期的影响。导致十六烷降解降低28%。所获得的用于实验的动力学方程式证实了固定化细菌对提高生物降解速率和降低土壤污染物半衰期的影响。导致十六烷降解降低28%。所获得的用于实验的动力学方程式证实了固定化细菌对提高生物降解速率和降低土壤污染物半衰期的影响。
更新日期:2020-01-21
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