In recent years, studies have found that in addition to nosocomial infections, methicillin-resistant Staphylococcus aureus (MRSA) can even cause community-associated infections, and MRSA spreads through food and animal that produce products in the community. In addition, MRSA can colonize the surface of food and food container and form biofilms by secreting extracellular polymers, leading to cross-infection. Cardamom essential oil has been found to be highly active against MRSA. Therefore, this study aimed to investigate the inhibitory effect of cardamom essential oil on MRSA biofilm and its mechanism. The semi-quantitative test results of crystal violet and the removal of MRSA biofilm on different food container materials show that cardamom essential oil can effectively remove MRSA biofilm and can be applied to the surface of food container materials. Meanwhile, the inhibition mechanism of cardamom essential oil on MRSA biofilm was studied by measuring the metabolic activity, extracellular polymer content and relative expression of MRSA biofilm formation related genes. Based on results, it is presumed that cardamom essential oil can function to scavenge MRSA biofilm by inhibiting the metabolic activity of bacteria, inhibiting the formation of extracellular polymers, and regulating the expression of genes involved in biofilm formation.

近年来的研究发现，除医院感染外，耐甲氧西林的金黄色葡萄球菌（MRSA）甚至可能导致社区相关的感染，并且MRSA通过在社区中生产产品的食物和动物传播。此外，MRSA可以在食物和食物容器的表面定殖，并通过分泌细胞外聚合物而形成生物膜，从而导致交叉感染。已经发现豆蔻精油对MRSA具有很高的活性。因此，本研究旨在探讨豆蔻精油对MRSA生物膜的抑制作用及其机理。结晶紫的半定量测试结果以及在不同食品容器材料上去除MRSA生物膜的结果表明，豆蔻精油可以有效地去除MRSA生物膜，并可以应用于食品容器材料的表面。与此同时，通过测量代谢活性，细胞外聚合物含量以及MRSA生物膜形成相关基因的相对表达，研究了豆蔻精油对MRSA生物膜的抑制机理。基于结果，推测豆蔻精油可通过抑制细菌的代谢活性，抑制细胞外聚合物的形成以及调节参与生物膜形成的基因的表达来清除MRSA生物膜。