Exopolysaccharides (EPS) synthesised by the tested strains of Lactobacillus rhamnosus bacteria (marked with symbols 0943, 0935 and OM-1) with probiotic properties are promising natural alternatives for commercial additives in the food, cosmetic and pharmaceutical industries. However, the low efficiency of synthesis and high acquisition cost make research and its industrial implementation much more difficult. In this paper, the environmental conditions and EPS extraction parameters were modelled. The culture medium used in the study was developed in the earlier stages of research on the basis of multi-parametric modelling. Thanks to a series of analyses, such cultivation parameters were developed which ensure high efficiency of EPS synthesis and a significantly reduced technological cost. The best results were obtained in stationary cultures cultivated for 24 h at 25 °C with the initial pH of 5.7 and the inoculum of 5% v/v. Unlike to other examined strains in case of Lb. rhamnosus ŁOCK 0943 prolongation of the incubation time and application of mixing (80 rpm) in the process of cultivation significantly increased the efficiency of EPS synthesis. The outcome sheds a new light on the possibility of the industrial application of the exopolysaccharides produced by Lb. rhamnosus bacteria in the “new generation” products.

由鼠李糖乳杆菌测试菌株合成的胞外多糖（EPS）具有益生菌性质的细菌（标有0943、0935和OM-1符号）是食品，化妆品和制药行业中商业添加剂的有希望的天然替代品。但是，合成效率低和购置成本高，使得研究及其工业实施更加困难。本文对环境条件和EPS提取参数进行了建模。本研究中使用的培养基是在多参数建模的基础上开发的。由于进行了一系列分析，开发了这样的培养参数，这些参数可确保EPS合成的高效率并显着降低技术成本。在25°C，初始pH值为5.7，接种量为5％v / v的固定培养物中培养24小时可获得最佳结果。磅。鼠李糖ŁOCK0943在培养过程中延长孵育时间和应用混合（80 rpm）显着提高了EPS合成的效率。结果为Lb产生的胞外多糖的工业应用提供了新的思路。鼠李糖杆菌细菌是“新一代”产品。