Trypanosoma cruzi, the protozoan agent of Chagas disease in the Americas, is comprised of six genetic lineages (TcI-TcVI) and a possible seventh (TcBat, related to TcI). Identification of T. cruzi lineages infecting reservoir mammalian species is fundamental to resolving transmission cycles. However, this is hindered by the limited sensitivity and technical complexity of parasite isolation and genotyping. An alternative approach is serology using T. cruzi lineage-specific epitopes, such as those of the trypomastigote small surface antigen (TSSA). For surveillance of T. cruzi lineage infections in mammal species from diverse Brazilian regions, we apply a novel rapid diagnostic test (RDT, Chagas Sero K-SeT), which incorporates the TSSA peptide epitope specific to TcII/V/VI (TSSApep-II/V/VI) and Protein G detection of antibodies. Chagas Sero K-SeT RDT results with sera from experimentally infected mice, from tamarin primates (Leontopithecus spp.) and from canines (Canis familiaris) were concordant with corresponding TSSApep-II/V/VI ELISAs. The Chagas Sero K-Set detected TcII/V/VI infections in Leontopithecus spp. from the Atlantic forest (n = 46), in C. familiaris (n = 16) and Thrichomys laurentius (n = 2) from Caatinga biome and Chiroptera (n = 1) from Acre, Amazonia. The Chagas Sero K-SeT RDT is directly applicable to TcII/V/VI-specific serological surveillance of T. cruzi infection in several different mammalian Orders. It can replace ELISAs and provides efficient, point-of-sampling, low-cost detection of TcII/V/VI infections, with at least equivalent sensitivity, although some mammals may be difficult to trap, and, not unexpectedly, Chagas Sero K-SeT could not recognise feline IgG. Knowledge of sylvatic hosts of T. cruzi can be expanded, new reservoir species discovered, and the ecology of transmission cycles clarified, particularly with adaptation to further mammalian Orders.

中文翻译：

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特定于谱系的快速诊断测试（Chagas Sero K-SeT）在不同的哺乳动物种群中鉴​​定出巴西克鲁氏锥虫II / V / VI的宿主。

美洲锥虫病的原生动物病原体克氏锥虫由六个遗传谱系（TcI-TcVI）和可能的第七个遗传谱系（TcBat，与TcI相关）组成。识别感染水库哺乳动物物种的克氏锥虫谱系是解决传播周期的基础。然而，这被寄生虫分离和基因分型的敏感性和技术复杂性所限制。另一种方法是使用克鲁氏锥虫谱系特异性表位（如锥虫小表面抗原（TSSA）的表位）进行血清学检查。为了监视巴西不同地区的哺乳动物中的克鲁氏锥虫谱系感染，我们应用了一种新颖的快速诊断测试方法（RDT，Chagas Sero K-SeT），该测试方法结合了特异于TcII / V / VI的TSSA肽表位（TSSApep-II / V / VI）和蛋白G抗体检测。Chagas Sero K-SeT RDT结果与实验感染的小鼠，猴灵长类动物（Leontopithecus spp。）和犬科动物（Canis熟悉）的血清与相应的TSSApep-II / V / VI ELISA一致。Chagas Sero K-Set在Leontopithecus spp中检测到TcII / V / VI感染。取自大西洋森林（n = 46），熟C鱼（n = 16）和取自Caatinga生物群系的Thrichomys laurentius（n = 2）和取自亚马逊河Acre的Chiroptera（n = 1）。Chagas Sero K-SeT RDT可直接应用于几种不同哺乳动物种群中针对TcII / V / VI的克鲁斯氏锥虫感染的血清学监测。它可以代替ELISA，并提供高效，采样点，低成本的TcII / V / VI感染检测，且灵敏度至少相当，尽管某些哺乳动物可能难以捕获，而且并非意料之外。Chagas Sero K-SeT无法识别猫IgG。可以扩展克氏锥虫的寄主宿主的知识，发现新的水库物种，并弄清传播周期的生态学，特别是在适应其他哺乳动物种群的情况下。