Swine leukocyte antigens play indispensable roles in immune responses by recognizing a large number of foreign antigens and thus, their genetic diversity plays a critical role in their functions. In this study, we developed a new high-resolution typing method for pig SLA-1 and successfully typed 307 individuals from diverse genetic backgrounds including 11 pure breeds, 1 cross bred, and 12 cell lines. We identified a total of 52 alleles including 18 novel alleles and 9 SLA-1 duplication haplotypes, including 4 new haplotypes. We observed significant differences in the distribution of SLA-1 alleles among the different pig breeds, including the breed specific alleles. SLA-1 duplication was observed in 33% of the chromosomes and was especially high in the biomedical model breeds such as SNU (100%) and NIH (76%) miniature pigs. Our analysis showed that SLA-1 duplication is associated with the increased level of SLA-1 mRNA expression in porcine cells compared to that of the single copy haplotype. Therefore, we provide here the results of the most extensive genetic analysis on pig SLA-1.

中文翻译：

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猪的 SLA-1 遗传多样性：拷贝数变异、杂合性、表达和品种特异性的广泛分析。

猪白细胞抗原通过识别大量外来抗原，在免疫反应中发挥着不可或缺的作用，因此，其遗传多样性对其功能起着至关重要的作用。在这项研究中，我们开发了一种新的猪 SLA-1 高分辨率分型方法，并成功对来自不同遗传背景的 307 个个体进行了分型，其中包括 11 个纯种、1 个杂交品种和 12 个细胞系。我们总共鉴定了 52 个等位基因，包括 18 个新等位基因和 9 个 SLA-1 重复单倍型，其中包括 4 个新单倍型。我们观察到不同猪品种之间 SLA-1 等位基因的分布存在显着差异，包括品种特异性等位基因。在 33% 的染色体中观察到 SLA-1 重复，在生物医学模型品种中尤其高，例如 SNU (100%) 和 NIH (76%) 小型猪。我们的分析表明，与单拷贝单倍型相比，SLA-1 重复与猪细胞中 SLA-1 mRNA 表达水平升高相关。因此，我们在此提供对猪 SLA-1 最广泛的遗传分析结果。