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A novel lncRNA as a positive regulator of carotenoid biosynthesis in Fusarium.
Scientific Reports ( IF 4.6 ) Pub Date : 2020-01-20 , DOI: 10.1038/s41598-020-57529-2
Obdulia Parra-Rivero 1 , Javier Pardo-Medina 1 , Gabriel Gutiérrez 1 , M Carmen Limón 1 , Javier Avalos 1
Affiliation  

The fungi Fusarium oxysporum and Fusarium fujikuroi produce carotenoids, lipophilic terpenoid pigments of biotechnological interest, with xanthophyll neurosporaxanthin as the main end product. Their carotenoid biosynthesis is activated by light and negatively regulated by the RING-finger protein CarS. Global transcriptomic analysis identified in both species a putative 1-kb lncRNA that we call carP, referred to as Fo-carP and Ff-carP in each species, upstream to the gene carS and transcribed from the same DNA strand. Fo-carP and Ff-carP are poorly transcribed, but their RNA levels increase in carS mutants. The deletion of Fo-carP or Ff-carP in the respective species results in albino phenotypes, with strong reductions in mRNA levels of structural genes for carotenoid biosynthesis and higher mRNA content of the carS gene, which could explain the low accumulation of carotenoids. Upon alignment, Fo-carP and Ff-carP show 75-80% identity, with short insertions or deletions resulting in a lack of coincident ORFs. Moreover, none of the ORFs found in their sequences have indications of possible coding functions. We conclude that Fo-carP and Ff-carP are regulatory lncRNAs necessary for the active expression of the carotenoid genes in Fusarium through an unknown molecular mechanism, probably related to the control of carS function or expression.

中文翻译:

新型lncRNA作为镰刀菌类胡萝卜素生物合成的正调控剂。

真菌尖孢镰刀菌(Fusarium oxysporum)和镰刀镰孢镰刀菌(Fusarium fujikuroi)生产类胡萝卜素,这是具有生物技术意义的亲脂性萜类色素,叶黄素神经孢子黄质是主要的最终产物。它们的类胡萝卜素生物合成被光激活,并受到RING手指蛋白CarS的负调控。全球转录组学分析在这两个物种中均确定了一个推定的1-kb lncRNA,我们将其称为carP,在每个物种中称为car-Car和Ff-carP,位于carS基因的上游,并从同一DNA链转录而成。Fo-carP和Ff-carP的转录较差,但在carS突变体中其RNA水平增加。各个物种中Fo-carP或Ff-carP的缺失导致白化病表型,类胡萝卜素生物合成的结构基因的mRNA水平大大降低,而carS基因的mRNA含量更高,这可以解释类胡萝卜素的低积累。对齐后,Fo-carP和Ff-carP显示出75-80%的同一性,短插入或缺失导致缺少一致的ORF。而且,在其序列中发现的ORF都没有指示可能的编码功能。我们得出结论,Fo-carP和Ff-carP是通过未知分子机制在镰刀菌中主动表达类胡萝卜素基因所必需的调控性lncRNA,可能与对carS功能或表达的控制有关。
更新日期:2020-01-21
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