While the effects of phytohormones on plant gene expression have been well characterized, comparatively little is known about how hormones influence metabolite profiles. This study examined the effects of elevated auxin and ethylene on the metabolome of Arabidopsis roots using a high-resolution 24 h time course, conducted in parallel to time-matched transcriptomic analyses. Mass spectrometry using orthogonal UPLC separation strategies (reversed phase and HILIC) in both positive and negative ionization modes was used to maximize identification of metabolites with altered levels. The findings show that the root metabolome responds rapidly to hormone stimulus and that compounds belonging to the same class of metabolites exhibit similar changes. The responses were dominated by changes in phenylpropanoid, glucosinolate, and fatty acid metabolism, although the nature and timing of the response was unique for each hormone. These alterations in the metabolome were not directly predicted by the corresponding transcriptome data, suggesting that post-transcriptional events such as changes in enzyme activity and/or transport processes drove the observed changes in the metabolome. These findings underscore the need to better understand the biochemical mechanisms underlying the temporal reconfiguration of plant metabolism, especially in relation to the hormone-metabolome interface and its subsequent physiological and morphological effects.

中文翻译：

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转录组的变化无法预测拟南芥根代谢组对植物生长素和乙烯的动态反应。

虽然植物激素对植物基因表达的作用已得到很好的表征，但对激素如何影响代谢物谱的了解相对较少。这项研究使用高分辨率的24 h时间过程，与时间匹配的转录组分析平行进行，研究了生长素和乙烯含量升高对拟南芥根代谢组的影响。使用在正电离和负电离模式下使用正交UPLC分离策略（反相和HILIC）进行的质谱分析可最大程度地鉴定水平变化的代谢物。研究结果表明，根代谢组对激素刺激反应迅速，属于同一类代谢产物的化合物表现出相似的变化。响应主要由苯丙烷，芥子油苷和脂肪酸代谢的变化决定，尽管每种激素的反应性质和时机都是独特的。相应的转录组数据并未直接预测代谢组中的这些变化，表明转录后事件（例如酶活性和/或转运过程的变化）推动了代谢组中观察到的变化。这些发现强调需要更好地了解植物代谢的时间重构的生化机制，特别是与激素-代谢组学界面及其随后的生理和形态学效应有关的机制。提示转录后事件，例如酶活性和/或转运过程的变化，推动了代谢组中观察到的变化。这些发现强调需要更好地了解植物代谢的时间重构的生化机制，特别是与激素-代谢组学界面及其随后的生理和形态学效应有关的机制。提示转录后事件，例如酶活性和/或转运过程的变化，推动了代谢组中观察到的变化。这些发现强调需要更好地了解植物代谢的时间重构的生化机制，特别是与激素-代谢组学界面及其随后的生理和形态学效应有关的机制。