Epilepsy is characterized by repeated spontaneous seizures and remains untreatable due to its complicate pathogenesis. Low-dose hydrogen sulfide (H₂S) has been shown to exert antiepileptic and protective effects in the central nervous system, but the administration of H₂S gas to suppress seizures is difficult. In the present study, we synthesized a safe and efficient carbazole-based H₂S donor from aldehydes with a one-pot procedure and characterized this specific H₂S donor via proton nuclear magnetic resonance (¹HNMR), scanning electron microscopy (SEM), and absorption spectroscopy. In vitro and in vivo risk assessments demonstrated that the H₂S donor (up to a 400-μM concentration) had sufficient biocompatibility and membrane permeability and suppressed epileptic seizures. Importantly, the suppression of seizures was determined in the brain when the H₂S donor was injected into the lateral ventricle in a rat model of advanced seizures. Furthermore, the mechanism by which the H₂S donor suppressed the expression of seizures may have been related to H₂S donor-induced increases in the expressions of the ATP-sensitive potassium channel (K_ATP) subunits, Kir6.2 and SUR1. Taken together, these assays suggested that our novel H₂S donor may represent a potential therapeutic strategy for ameliorating seizures in epilepsy.

癫痫病的特征是反复自发发作，并且由于其复杂的发病机理而无法治疗。低剂量的硫化氢（H ₂ S）已被证明在中枢神经系统中具有抗癫痫和保护作用，但难以抑制H ₂ S气体的给药。在本研究中，我们通过一锅法从醛中合成了一种安全有效的基于咔唑的H ₂ S供体，并通过质子核磁共振（¹ HNMR），扫描电子显微镜（SEM）对这种特定的H ₂ S供体进行了表征。 ，和吸收光谱。体外和体内风险评估表明，H ₂ S供体（浓度高达400μM）具有足够的生物相容性和膜通透性，并抑制了癫痫发作。重要的是，在晚期癫痫的大鼠模型中，当将H ₂ S供体注入侧脑室时，可以确定大脑中癫痫的抑制作用。此外，H ₂ S供体抑制癫痫发作表达的机制可能与H ₂ S供体诱导的ATP敏感性钾通道（K _ATP）亚基Kir6.2和SUR1的表达增加有关。综上所述，这些测定表明我们新颖的H ₂S供体可能代表减轻癫痫发作的潜在治疗策略。