The plethora of optical techniques have been developed to detect the dengue virus, regrettably, the existing techniques are cumbersome and have limitations to provide early identification of the low-level of dengue virus. For this purpose, surface plasmon resonance (SPR) optical sensor was designed with a highly sensitive thin film based on hyperbranched polymer nanocomposites for conjugation of antibodies that specifically detect the dengue virus type II (DENV). In particular, we investigate the SPR sensing performance for gold/cadmium sulfide quantum dots-polyamidoamine/antibody (Au/CdS QDs-PAMAM/IgM) sensor film in terms of linear sensitivity, binding affinity, detection accuracy, full-width-half-maximum, selectivity, and spike and recovery test. The results show a strong affinity value, good sensitivity and selectivity, high accuracy, and excellent recovery value, obeying the ideal value based on conventional assay analysis. Additionally, this Au/CdS QDs-PAMAM/IgM based SPR sensor was successfully applied for the lowest detection of 1 pM of DENV E-proteins. To this end, a highly sensitive Au/CdS QDs-PAMAM/IgM sensor film was characterized using Raman spectroscopy, ultraviolet-visible (UV–Vis-NIR) spectroscopy, and atomic force microscopy (AFM) to confirm the binding of DENV.

已经开发了多种光学技术来检测登革热病毒，令人遗憾的是，现有技术繁琐并且具有局限性，无法提供对低水平登革热病毒的早期鉴定。为此，设计了基于超支化聚合物纳米复合材料的高敏感度薄膜的表面等离振子共振（SPR）光学传感器，用于缀合特异性检测II型登革病毒（DENV）的抗体。特别是，我们从线性灵敏度，结合亲和力，检测准确度，半角全宽等方面研究了金/硫化镉量子点-聚酰胺酰胺/抗体（Au / CdS QDs-PAMAM / IgM）传感器膜的SPR传感性能。最大，选择性以及加标和恢复测试。结果显示亲和力强，灵敏度和选择性好，精度高，并具有出色的回收率，遵循常规测定分析的理想值。此外，基于Au / CdS QDs-PAMAM / IgM的SPR传感器已成功应用于最低1 pM DENV E蛋白的检测。为此，使用拉曼光谱，紫外可见（UV-Vis-NIR）光谱和原子力显微镜（AFM）对高灵敏度的Au / CdS QDs-PAMAM / IgM传感器膜进行了表征，以确认DENV的结合。