Neutrophils are the most abundant leukocytes in human blood and critical actors of the immune system. Many neutrophil functions and facets of their activity in vivo were revealed by studying genetically modified mice or by tracking fluorescent neutrophils in animals using imaging approaches. Assessing the roles of neutrophils can be challenging, especially when exact molecular pathways are questioned or disease states are interrogated that alter normal neutrophil homeostasis. This review discusses the main in vivo models for the study of neutrophils, their advantages and limitations. The side-by-side comparison underlines the necessity to carefully choose the right model(s) to answer a given scientific question, and exhibit caveats that need to be taken into account when designing experimental procedures. Collectively, this review suggests that at least two models should be employed to legitimately conclude on neutrophil functions.

中文翻译：

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嗜中性粒细胞体内研究的小鼠模型和工具。

中性粒细胞是人类血液中最丰富的白细胞，也是免疫系统的关键角色。通过研究转基因小鼠或通过使用成像方法追踪动物中的荧光中性粒细胞，揭示了许多中性粒细胞功能和体内活性方面。评估中性粒细胞的作用可能具有挑战性，尤其是在质疑确切的分子途径或询问疾病状态以改变正常的中性粒细胞稳态时。本文综述了研究嗜中性粒细胞的主要体内模型，它们的优点和局限性。并排比较强调了必须仔细选择正确的模型来回答给定的科学问题的必要性，并展示设计实验程序时需要考虑的警告。总的来说，