In this study, we investigated the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v⁻¹) using a central composite rotatable design. Sonication time (10 to 330 s), US intensity (120.6 to 263.7 W cm⁻²), and reaction temperature (25 to 50 °C) were varied using a horn-type ultrasound reactor, and endoglucanase (CMCase) and total cellulase (FPase) activities were determined. US intensity had a positive effect on enzyme activity. Under optimal conditions (170 s, 180.8 W cm⁻², and 25 °C), CMCase activity was 13% higher than that of the control. In the presence of substrate, CMCase activity increased by 33.87% and K_M reduced by 23% in relation to that of the control. The theoretical yield of cellulose was 42.08%. Cellulase activity can be improved by US treatment to maximize productivity gains and reduce costs in second-generation ethanol production, by the action of a low-frequency ultrasound with a short ultrasonication time of application.

在这项研究中，我们研究了使用中央复合材料在不存在和存在纤维素底物（Avicel®，2％wv ^-1）的情况下，超声（US）对商业纤维素酶（Celluclast®1.5 L）活性的影响。可旋转的设计。使用喇叭型超声反应器，内切葡聚糖酶（CMCase）和总纤维素酶（10-330 s），超声强度（120.6-263.7 W cm ^-2）和反应温度（25-50°C）进行超声处理确定了FPase）活性。美国强度对酶活性有积极影响。在最佳条件下（170 s，180.8 W cm ^-2和25°C），CMCase活性比对照高13％。在底物存在下，CMCase活性和K _M分别增加33.87％与对照组相比减少了23％。纤维素的理论收率为42.08％。纤维素酶活性可以通过美国的处理得以改善，从而通过低频超声和较短的超声应用时间来发挥作用，从而最大限度地提高生产率并降低第二代乙醇生产的成本。