Macrophages within tissues display a strong plastic ability in respond to environmental cues in both physiologic influences and disease. However, the macrophage phenotype and its distribution in the bone marrow biopsies (BMB) samples of human acute leukemia (AL) remain poorly understood. In this study, 97 BMB samples of patients with acute leukemia and 30 iron-deficiency anemias (IDA) as control group were evaluated with immunohistochemistry. In comparison with controls, the counts of CD68+, CD163+, and CD206+macrophages were remarkably increased in BMB samples of acute leukemia (P < 0.01), as well as their infiltration density was roaring up-regulation (P < 0.01). The expression levels of CD68+, CD163+, and CD206+macrophages were decreased in patients with complete remission, but there still existed statistically significant contrast to the control group (P < 0.01). The ratios of the CD163-positive cells or CD206-positive cells to CD68-positive cells were most prevalent in the BMB samples of human acute leukemia compared with the control group (P < 0.01), which support that macrophages were polarized to M2 macrophages.

中文翻译：

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人类急性白血病BMB样品中巨噬细胞的表型表征。

组织中的巨噬细胞在生理影响和疾病方面都对环境提示有很强的可塑性。然而，人类急性白血病（AL）的骨髓活检（BMB）样本中的巨噬细胞表型及其分布仍然知之甚少。在这项研究中，采用免疫组织化学方法评估了97例急性白血病和30例缺铁性贫血（IDA）患者的BMB样本。与对照组相比，急性白血病的BMB样本中CD68 +，CD163 +和CD206 +巨噬细胞的计数显着增加（P <0.01），并且其浸润密度正上调（P <0.01）。完全缓解的患者中CD68 +，CD163 +和CD206 +巨噬细胞的表达水平降低，但与对照组相比仍存在统计学上的显着差异（P <0.01）。与对照组相比，人急性白血病的BMB样品中CD163阳性细胞或CD206阳性细胞与CD68阳性细胞的比率最普遍（P <0.01），这支持巨噬细胞极化为M2巨噬细胞。