The studies on natural compounds to diabetes mellitus treatment have been increasing in recent years. Research suggests that natural components can inhibit alpha-glucosidase activities, an important strategy in the management of blood glucose levels. In this work, for the first time in the literature, the compounds produced by Ganoderma lipsiense extracts were identified and evaluated on the inhibitory effect of these on alpha-glucosidase activity. Four phenolic compounds were identified by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) to crude extract from G. lipsiense grown in red rice medium (RCE) and synthetic medium (SCE), being syringic acid identified in both extracts. Gas chromatography-mass spectrometry (GC-MS) analysis showed fatty acids and their derivatives, terpene, steroid, niacin, and nitrogen compounds to SCE, while RCE was rich in fatty acids and their derivatives. Both extracts demonstrated alpha-glucosidase inhibition (RCE IC50 = 0.269 ± 8.25 mg mL-1; SCE IC50 = 0.218 ± 9.67 mg mL-1), and the purified hexane fraction of RCE (RHEX) demonstrated the highest inhibition of enzyme (81.1%). Studies on kinetic inhibition showed competitive inhibition mode to RCE, while SCE showed uncompetitive inhibition mode. Although the inhibitory effects of RCE and SCE were satisfactory, the present findings identified some unpublished compounds to G. lipsiense in the literature with important therapeutic properties.

中文翻译：

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α-葡糖苷酶抑制作用的动力学分析和脂灵芝菌丝体化合物的鉴定。

近年来，关于天然化合物治疗糖尿病的研究一直在增加。研究表明，天然成分可以抑制α-葡萄糖苷酶活性，这是控制血糖水平的重要策略。在这项工作中，这是文献中的首次，是鉴定和鉴定了由灵芝提取物产生的化合物对这些化合物对α-葡萄糖苷酶活性的抑制作用。通过高效液相色谱-电喷雾串联质谱法（HPLC-ESI-MS / MS）从红米培养基（RCE）和合成培养基（SCE）中生长出的唇纹假单胞菌的粗提物中鉴定出4种酚类化合物两种提取物中均含有酸。气相色谱-质谱（GC-MS）分析表明，脂肪酸及其衍生物，萜烯，类固醇，烟酸和含氮化合物转化为SCE，而RCE富含脂肪酸及其衍生物。两种提取物均显示出α-葡萄糖苷酶抑制作用（RCE IC50 = 0.269±8.25 mg mL-1; SCE IC50 = 0.218±9.67 mg mL-1），RCE的纯化己烷级分（RHEX）显示出对酶的最高抑制作用（81.1％ ）。动力学抑制研究表明对RCE具有竞争性抑制模式，而SCE具有非竞争性抑制模式。尽管RCE和SCE的抑制作用令人满意，但目前的发现在文献中发现了一些尚未发表的对唇纹假单胞菌具有重要治疗作用的化合物。RCE（RHEX）的纯化己烷馏分显示出最高的酶抑制率（81.1％）。动力学抑制研究表明对RCE具有竞争性抑制模式，而SCE具有非竞争性抑制模式。尽管RCE和SCE的抑制作用令人满意，但目前的发现在文献中发现了一些尚未发表的对唇纹假单胞菌具有重要治疗作用的化合物。RCE（RHEX）的纯化己烷馏分显示出最高的酶抑制率（81.1％）。动力学抑制研究表明对RCE具有竞争性抑制模式，而SCE具有非竞争性抑制模式。尽管RCE和SCE的抑制作用令人满意，但目前的发现在文献中发现了一些尚未发表的对唇纹假单胞菌具有重要治疗作用的化合物。