Objectives

Biphasic calcium phosphates (BCP) are synthetic biomaterials developed as an alternative to the autogenous bone grafts and xenografts. The aim of the present study was to assess the influence of the addition of collagen onto the BCP resorption rate and bone formation.

Material and methods

Eighteen male NWZ rabbits approximately 12 weeks of age were used. Critical size defects were randomly treated with bilayered BCP materials comprising 12% HA and 88% α-TCP with and without collagen or sham-operated, respectively. All defects were covered with a resorbable collagen membrane. Animals were euthanized after 3 and 12 weeks of healing and investigated by micro-CT, histologic, and histomorphometric analysis.

Results

Woven bone formation was observed from the original bone at 3-week healing in all samples. After 3 months, mainly lamellar new bone in the peripheral area was observed. In the central region, both woven and lamellar bone were seen. Samples containing collagen showed less residual biomaterial than without collagen at both healing periods. Both types of granules were in close contact with new bone, yielding a complete defect closure at 3 months of healing. However, new bone volume and area was similar for both biomaterials.

Conclusions

Within its limitations, the study results qualify collagen as a biocompatible carrier for BCPs. The presence of collagen indicated neither significant impact on the resorption of the BCPs nor on bone formation.

Clinical relevance

The addition of collagen to BCPs might not be beneficial for the augmentation of extended bone deficiencies.

中文翻译：

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双相磷酸钙中其他胶原蛋白的作用：在兔颅盖骨中的一项研究。

目标

双相磷酸钙（BCP）是合成生物材料，可替代自体骨移植物和异种移植物。本研究的目的是评估添加胶原蛋白对BCP吸收速率和骨形成的影响。

材料与方法

使用了大约12周龄的18只雄性NWZ兔子。临界尺寸缺损用含有B％HA和88％α-TCP的双层BCP材料（有和没有胶原蛋白或假手术）随机处理。所有缺陷均被可吸收胶原膜覆盖。在愈合3周和12周后对动物实施安乐死，并通过micro-CT，组织学和组织形态学分析对其进行研究。

结果

在所有样品中，在愈合3周后均观察到从原始骨骼形成的编织骨骼。3个月后，在周边区域主要观察到层状新骨。在中部地区，可以看到编织骨和层状骨。在两个愈合期中，含有胶原蛋白的样品显示出的残留生物材料要比不含胶原蛋白的样品少。两种类型的颗粒都与新骨紧密接触，在愈合的3个月内完全闭合了缺损。但是，两种生物材料的新骨量和面积均相似。

结论

在其限制范围内，研究结果使胶原蛋白成为BCP的生物相容性载体。胶原蛋白的存在对BCP的吸收和骨形成均无明显影响。

临床相关性

向BCP中添加胶原蛋白可能不利于扩大骨缺损。