High temperature promotes guard cell expansion, which opens stomatal pores to facilitate leaf cooling. How the high-temperature signal is perceived and transmitted to regulate stomatal aperture is, however, unknown. Here, we used a reverse-genetics approach to understand high temperature-mediated stomatal opening in Arabidopsis (Arabidopsis thaliana). Our findings reveal that high temperature-induced guard cell movement requires components involved in blue light-mediated stomatal opening, suggesting cross talk between light and temperature signaling pathways. The molecular players involved include phototropin photoreceptors, plasma membrane H+-ATPases, and multiple members of the 14-3-3 protein family. We further show that phototropin-deficient mutants display impaired rosette evapotranspiration and leaf cooling at high temperatures. Blocking the interaction of 14-3-3 proteins with their client proteins severely impairs high temperature-induced stomatal opening but has no effect on the induction of heat-sensitive guard cell transcripts, supporting the existence of an additional intracellular high-temperature response pathway in plants.

中文翻译：

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保卫细胞整合光和温度信号以控制气孔孔径。

高温会促进保卫细胞膨胀，从而打开气孔，促进叶片冷却。然而，如何感知和传输高温信号以调节气孔孔径尚不清楚。在这里，我们使用反向遗传学方法来了解拟南芥（Arabidopsis thaliana）中高温介导的气孔开放。我们的发现表明，高温诱导的保卫细胞运动需要参与蓝光介导的气孔开放的成分，这表明光和温度信号通路之间存在串扰。涉及的分子包括光养蛋白光感受器，质膜H + -ATPase和14-3-3蛋白家族的多个成员。我们进一步表明光合蛋白缺乏突变体显示受损的玫瑰花蒸散量和高温下的叶片冷却。