The multifunctional hemin@carbon dot hybrid nanozymes (hemin@CD) with simultaneous peroxidase-like activity and fluorescence signalling property was prepared for the first time. Based on these properties, hemin@CD was applied to develop a dual-channel fluorescent probe for H 2 O 2 and H 2 O 2 -based biocatalytic systems. By virtue of the peroxidase-like activity, hemin@CD can catalyze the oxidative coupling of 4-aminoantipyrine with phenol in the presence of H 2 O 2 to form a pink-red quinoneimine dye with a maximum absorbance at 505 nm. Under the excitation wavelength of 480 nm, the green fluorescence of hemin@CD peaks at 540 nm and is quenched by the generated quinoneimine dye due to an inner filter effect, and also by H 2 O 2 because of dynamic quenching. Thus, a colorimetric and fluorimetric dual-channel optical probe for H 2 O 2 is obtained. Due to the glucose/xanthine transformations under formation of H 2 O 2 by the relevant oxidase catalysis, the probe can be applied for detection of glucose and xanthine. The colorimetric detection limits for H 2 O 2 , glucose and xanthine are 0.11, 0.15, 0.11 μM, and the and fluorimetric detection limits are 0.15, 0.15, 0.12 μM, respectively. Graphical abstract Schematic representation of the colorimetric and fluorimetric dual probe for H 2 O 2 , glucose and xanthine based on the multifunctional emin@carbon dot) hybrid nanozymes with simultaneous peroxidase-like activity and fluorescence signalling property.

中文翻译：

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Hemin@carbon dot 杂化纳米酶具有过氧化物酶模拟特性，可用于过氧化氢、葡萄糖和黄嘌呤的双重（比色和荧光）传感

首次制备了同时具有类过氧化物酶活性和荧光信号特性的多功能 hemin@carbon dot 杂化纳米酶（hemin@CD）。基于这些特性，hemin@CD 被用于开发用于 H 2 O 2 和 H 2 O 2 基生物催化系统的双通道荧光探针。凭借类似过氧化物酶的活性，hemin@CD 可以在 H 2 O 2 存在下催化 4-氨基安替比林与苯酚的氧化偶联，形成粉红色的醌亚胺染料，在 505 nm 处具有最大吸光度。在 480 nm 激发波长下，hemin@CD 的绿色荧光在 540 nm 处达到峰值，由于内部滤光效应而被生成的醌亚胺染料猝灭，也被 H 2 O 2 因动态猝灭而猝灭。因此，获得了用于H 2 O 2 的比色和荧光双通道光学探针。由于在相关氧化酶催化下形成H 2 O 2 下的葡萄糖/黄嘌呤转化，该探针可用于检测葡萄糖和黄嘌呤。H 2 O 2 、葡萄糖和黄嘌呤的比​​色检测限为0.11、0.15、0.11 μM，和荧光检测限分别为0.15、0.15、0.12 μM。图形摘要 基于多功能 emin@carbon dot) 杂化纳米酶的 H 2 O 2 、葡萄糖和黄嘌呤的比​​色和荧光双探针的示意图，同时具有过氧化物酶样活性和荧光信号传导特性。11、0.15、0.11 μM，荧光检测限分别为0.15、0.15、0.12 μM。图形摘要 基于多功能 emin@carbon dot) 杂化纳米酶的 H 2 O 2 、葡萄糖和黄嘌呤的比​​色和荧光双探针的示意图，同时具有过氧化物酶样活性和荧光信号传导特性。11、0.15、0.11 μM，荧光检测限分别为0.15、0.15、0.12 μM。图形摘要 基于多功能 emin@carbon dot) 杂化纳米酶的 H 2 O 2 、葡萄糖和黄嘌呤的比​​色和荧光双探针的示意图，同时具有过氧化物酶样活性和荧光信号传导特性。