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Characterization of tetracycline effects on microbial community, antibiotic resistance genes and antibiotic resistance of Aeromonas spp. in gut of goldfish Carassius auratus Linnaeus.
Ecotoxicology and Environmental Safety ( IF 6.8 ) Pub Date : 2020-01-17 , DOI: 10.1016/j.ecoenv.2020.110182
Jia Jia 1 , Mengqian Cheng 1 , Xue Xue 1 , Yongjing Guan 1 , Zaizhao Wang 1
Affiliation  

The gut of aquatic animals was a significant niche for dissemination of antibiotic resistance genes (ARGs) and direct response of living conditions. In this study, the gut microbiota of goldfish Carassius auratus Linnaeus was sampled at 7 days and 21 days after treatment with tetracycline at 0.285 and 2.85 μg L-1 to investigate the influences on the microbial structure and antibiotic resistance. The proportion of tetracycline resistance bacteria was 1.02% in the control group, while increased to 23.00%, 38.43%, 62.05% in groups of high concentration for 7 days (H7), low concentration for 21 days (L21) and high concentration for 21 days (H21), respectively. Compared to the control group, the diversity of isolated Aeromonas spp. was decreased in the treatment groups and the minimal inhibitory concentration (MIC) of resistant isolates was enhanced from 32 to 256 μg mL-1 with the treatment of tetracycline in time- and dose-dependent manners. Furthermore, the abundance of most genes was increased in treatment groups and efflux genes mainly responded to the stress of tetracycline with an average level of 1.0 × 10-2. After treatment with tetracycline, the predominant species were changed both at phylum and genus levels. The present study explored the impact of tetracycline on gut microbiota of goldfish at environmentally realistic concentrations for the first time and our findings will provide a reference for characterizing the microbiome of fish in the natural environment.

中文翻译:

表征四环素对微生物群落,抗生素抗性基因和气单胞菌的抗性的影响。在金鱼Car鱼肠内。

水生动物的肠道是传播抗生素抗性基因(ARG)和生活条件直接反应的重要利基。在这项研究中,金鱼Car鱼的肠道菌群在用0.285和2.85μgL-1的四环素处理后第7天和第21天取样,以研究其对微生物结构和抗生素抗性的影响。对照组中四环素抗性细菌的比例为1.02%,而高浓度7天(H7),低浓度21天(L21)和高浓度21天的组分别增加到23.00%,38.43%,62.05%。天(H21)。与对照组相比,分离的气单胞菌属物种的多样性。在四环素处理中,以时间和剂量依赖性方式将治疗组的抗药性降低,并将耐药菌的最低抑菌浓度(MIC)从32μgmL-1提高至256μgmL-1。此外,治疗组中大多数基因的丰度增加,外排基因主要对四环素的胁迫作出反应,平均水平为1.0×10-2。用四环素处理后,主要种类在门和属水平上都发生了变化。本研究首次探索了四环素对环境中实际浓度的金鱼肠道菌群的影响,我们的发现将为表征自然环境中鱼的微生物组提供参考。治疗组大多数基因的丰度增加,外排基因主要对四环素的应激反应,平均水平为1.0×10-2。用四环素处理后,主要种类在门和属水平上都发生了变化。本研究首次探索了四环素对环境中实际浓度的金鱼肠道菌群的影响,我们的发现将为表征自然环境中鱼的微生物组提供参考。治疗组大多数基因的丰度增加,外排基因主要对四环素的应激反应,平均水平为1.0×10-2。用四环素处理后,主要种类在门和属水平上都发生了变化。本研究首次探索了四环素对环境中实际浓度的金鱼肠道菌群的影响,我们的发现将为表征自然环境中鱼的微生物组提供参考。
更新日期:2020-01-17
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