Abstract Chromium picolinate (CrPic) and trace hexavalent chromium (Cr(VI)) content were characterized in VITA-1 and VITB-1, two new multivitamin and mineral supplement candidate reference materials from National Research Council Canada, by two methods of high performance liquid chromatography inductively-coupled plasma triple quadrupole mass spectrometry. The conditions for the separation of Cr(VI) from EDTA-complexed Cr(III) were optimized such that species interconversions were not observed during analysis. Following extraction with dilute NH4OH in water at pH 10 (for Cr(VI)) or 3:2 acetonitrile:water (for CrPic) and quantification by standard addition, it was concluded that CrPic accounted for 95–96 % of the Cr in VITA-1 and VITB-1, while Cr(VI) was not present above the detection limit of 0.13 μg Cr g−1. Using the two developed methodologies, commercially-available nutritional supplements, in the form of vitamin tablets and dried food supplements, were assessed, and it was determined that CrPic accounted for the majority (92 %–98 %) of the chromium present in the tablets, and that Cr(VI) concentrations were below the detection limit for all samples except for the powdered beets where it accounted for nearly half of the total Cr present.

中文翻译：

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HPLC-ICP-MS/MS 测定多种维生素和补充剂中的吡啶甲酸铬和痕量六价铬

摘要 通过两种高效液相色谱法，对来自加拿大国家研究委员会的两种新型复合维生素和矿物质补充剂候选参考物质 VITA-1 和 VITB-1 中吡啶甲酸铬 (CrPic) 和痕量六价铬 (Cr(VI)) 的含量进行了表征。色谱电感耦合等离子体三重四极杆质谱。对从 EDTA 络合的 Cr(III) 中分离 Cr(VI) 的条件进行了优化，以便在分析过程中未观察到物种相互转化。在 pH 值为 10（对于 Cr(VI)）或 3:2 乙腈:水（对于 CrPic）的水中用稀 NH4OH 萃取并通过标准添加进行定量后，得出的结论是，CrPic 占 VITA 中 Cr 的 95-96% -1 和 VITB-1，而 Cr(VI) 的含量不超过 0.13 μg Cr g-1 的检测限。使用两种已开发的方法，