Vascular smooth muscle cells (VSMCs) can be derived in large numbers from human induced pluripotent stem cells (hiPSCs) for producing tissue-engineered vascular grafts (TEVGs). However, hiPSC-derived TEVGs are hampered by low mechanical strength and significant radial dilation after implantation. Here, we report generation of hiPSC-derived TEVGs with mechanical strength comparable to native vessels used in arterial bypass grafts by utilizing biodegradable scaffolds, incremental pulsatile stretching, and optimal culture conditions. Following implantation into a rat aortic model, hiPSC-derived TEVGs show excellent patency without luminal dilation and effectively maintain mechanical and contractile function. This study provides a foundation for future production of non-immunogenic, cellularized hiPSC-derived TEVGs composed of allogenic vascular cells, potentially serving needs to a considerable number of patients whose dysfunctional vascular cells preclude TEVG generation via other methods.

中文翻译：

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来自人类iPSC的具有先进机械强度的组织工程化血管移植物。

血管平滑肌细胞（VSMC）可以大量来源于人诱导的多能干细胞（hiPSC），用于生产组织工程化的血管移植物（TEVG）。但是，hiPSC衍生的TEVG受较低的机械强度和植入后明显的径向扩张的阻碍。在这里，我们报告了利用可生物降解的支架，增加的搏动性拉伸和最佳的培养条件，产生了具有可与动脉旁路移植术中使用的天然血管媲美的机械强度的hiPSC衍生的TEVG。植入大鼠主动脉模型后，hiPSC衍生的TEVG表现出出色的通畅性，而没有管腔扩张，并有效地维持了机械和收缩功能。这项研究为以后生产非免疫原性，细胞化的hiPSC衍生的TEVGs（由同种异体血管细胞组成）奠定了基础，