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A novel electrochemical immunosensor for ultrasensitive detection of CA125 in ovarian cancer.
Biosensors and Bioelectronics ( IF 12.6 ) Pub Date : 2020-01-16 , DOI: 10.1016/j.bios.2020.112029
Parvin Samadi Pakchin 1 , Marziyeh Fathi 2 , Hossein Ghanbari 3 , Reza Saber 4 , Yadollah Omidi 5
Affiliation  

In the current study, we report on the design and development of a novel electrochemical immunosensor for the detection of cancer antigen 125 (CA125) oncomarker. Polyamidoamine/gold nanoparticles (PAMAM/AuNPs) were used to increase the conductivity and enhance the number of antibodies (Abs) immobilized on the electrode surface. Three-dimensional reduced graphene oxide-multiwall carbon nanotubes (3DrGO-MWCNTs) were used to modify the glassy carbon electrode to improve the electrode conductivity and specific surface area. Ab and toluidine blue attached to O-succinyl-chitosan-magnetic nanoparticles (Suc-CS@MNPs) as a tracer. The poor solubility of chitosan (CS) was improved by succinic anhydride using a novel modification method. Under optimum condition, the developed immunosensor exhibited a wide linear range (0.0005-75 U/mL) and an excellent limit of detection around 6 μU/mL. The reliability of the engineered immunosensor in detecting CA125 was verified by standard addition recovery method, which was further compared to enzyme-linked immunosorbent assay (ELISA). The proposed immunosensor exhibited excellent stability, high selectivity and sensitivity, and good reproducibility. Based on the great performance of the engineered immunosensor, it is proposed as a robust and reliable diagnostic tool for the detection of CA125 in the clinic.

中文翻译:

一种用于卵巢癌中CA125超灵敏检测的新型电化学免疫传感器。

在当前的研究中,我们报告了一种新型电化学免疫传感器的设计和开发,该传感器可用于检测结肠癌中的癌症抗原125(CA125)。聚酰胺基胺/金纳米颗粒(PAMAM / AuNPs)用于增加电导率并增加固定在电极表面的抗体(Abs)的数量。三维还原氧化石墨烯-多壁碳纳米管(3DrGO-MWCNTs)用于修饰玻碳电极,以提高电极电导率和比表面积。Ab和甲苯胺蓝附着在O-琥珀酰-壳聚糖磁性纳米颗粒(Suc-CS @ MNPs)上作为示踪剂。壳聚糖(CS)的不良溶解性通过使用新型改性方法的琥珀酸酐得以改善。在最佳条件下,开发的免疫传感器表现出较宽的线性范围(0。0005-75 U / mL)和6μU/ mL左右的极佳检测限。通过标准添加回收方法验证了工程免疫传感器检测CA125的可靠性,并将其与酶联免疫吸附测定(ELISA)进行了进一步比较。所提出的免疫传感器表现出优异的稳定性,高选择性和灵敏度以及良好的再现性。基于工程免疫传感器的出色性能,它被建议作为一种可靠且可靠的诊断工具,用于在临床中检测CA125。重复性好。基于工程免疫传感器的出色性能,它被建议作为一种可靠且可靠的诊断工具,用于在临床中检测CA125。重复性好。基于工程免疫传感器的出色性能,它被建议作为一种可靠且可靠的诊断工具,用于在临床中检测CA125。
更新日期:2020-01-17
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