Background

Iinflammatory bowel disease (IBD) is a chronic disease characterised by dysregulation of the immune function response and imbalanced release of cytokines and unresolved inflammatory progress associated with the intestinal mucosa. In this way, new drugs are constantly tested as a novel treatment option. Mimosa caesalpiniifolia (MC) has been described as a potent analgesic, anti-inflammatory, anti-haemorrhagic, astringent, anthelmintic and diuretic. This study aimed to evaluate the effect of MC extract on dextran sulphate sodium (DSS)-induced colitis in Wistar rats.

Methods

Forty male Wistar rats were randomised into five groups: Sham; 5% DSS-induced colitis; control MC extract (125 mg/kg/day); 5% DSS-induced colitis treated with MC (125 mg/kg/day); 5% DSS-induced colitis treated with MC + 5-ASA (125 mg/kg/day for both). Colitis was induced by administration of 5% DSS (MP Biomedicals), diluted in drinking water and offered ad libitum for 5 days. MC plant extract was diluted in water and administered by oral gavage per 6 days. The leaf extracts were prepared in 70 gl ethanol and dried in a Buchi B19 Mini-spray dryer using 20% aerosol. The selection of the operating conditions used was followed by the Laboratory of Medicinal and Phytotherapeutic Plants of Unifal-MG. After the induction of colitis, body weight, general health status and faecal characteristics will be evaluated daily. All rats were euthanised on the ninth day of the experiment. The entire colon was dissected and fixed in 10% neutral-buffered formalin at room temperature and embedded in paraffin to provide sections for histological evaluation (Ethics Committee on the Use of Animals number 4362180119). The severity of colitis was evaluated in sections stained with Hematoxylin and Eosin. Parameters such as inflammation extent, regeneration and crypt damage were graded according to Dieleman et al. Statistical analysis was performed by one-way analysis of variance (ANOVA), followed by Tukey post hoc test using Graph Pad Prism (version 6.0).

Results

MC extract treatment reduced significantly the severity of DSS-induced colitis evidenced by a decreased in clinical symptoms (p < 0.0001). The rats treated with MC extract showed a decreased in diarrhoea episodes and rectal bleeding. We observed histopathological changes of colon tissue between the DSS-treatment and control groups, however, without statistical significance. The MC extract treatment reduced the damage to the colonic tissue evidenced by a decreased of inflammatory cells, ulcerations, and goblet cell preservation.

Conclusion

MC extract reduces the clinical signs and suggests a potential anti-inflammatory effect on DSS-induced colitis.

中文翻译：

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P055含羞草提取物对Wistar大鼠DSS诱发的结肠炎的作用

背景

肠炎性肠病（IBD）是一种慢性疾病，其特征在于免疫功能反应失调，细胞因子释放失衡以及与肠粘膜相关的未解决的炎症进展。通过这种方式，不断对新药进行测试，并将其作为一种新颖的治疗选择。含羞草含羞草（MC）被描述为有效的止痛，消炎，止血，收敛，驱虫药和利尿药。这项研究旨在评估MC提取物对Wistar大鼠硫酸葡聚糖硫酸钠（DSS）诱导的结肠炎的作用。

方法

将四十只雄性Wistar大鼠随机分为五组：假手术组；假手术组。5％DSS诱发的结肠炎；对照MC提取物（125 mg / kg /天）; 5％DSS诱导的结肠炎，用MC治疗（125 mg / kg /天）；5％DSS诱导的结肠炎，用MC + 5-ASA（两种药物均为125 mg / kg /天）治疗。通过施用5％DSS（MP Biomedicals）诱导结肠炎，将其在饮用水中稀释并随意提供5天。将MC植物提取物在水中稀释并每6天通过口服管饲法施用。叶提取物在70毫升乙醇中制备，并在Buchi B19小型喷雾干燥器中使用20％气雾剂干燥。Unifal-MG的药用和植物治疗实验室负责选择使用的操作条件。诱发结肠炎后，将每天评估体重，总体健康状况和粪便特征。在实验的第九天将所有大鼠安乐死。解剖整个结肠，并在室温下固定在10％中性缓冲的福尔马林中，并包埋在石蜡中，以提供切片进行组织学评估（动物使用伦理委员会编号4362180119）。在用苏木精和曙红染色的切片中评估结肠炎的严重程度。诸如炎症程度，再生和隐窝损害等参数根据Dieleman等人的方法分级。通过单向方差分析（ANOVA）进行统计分析，然后使用Graph Pad Prism（6.0版）进行Tukey post hoc测试。解剖整个结肠，并在室温下固定在10％中性缓冲的福尔马林中，并包埋在石蜡中，以提供切片进行组织学评估（动物使用伦理委员会编号4362180119）。在用苏木精和曙红染色的切片中评估结肠炎的严重程度。诸如炎症程度，再生和隐窝损害等参数根据Dieleman等人的方法分级。通过单向方差分析（ANOVA）进行统计分析，然后使用Graph Pad Prism（6.0版）进行Tukey post hoc测试。解剖整个结肠，并在室温下固定在10％中性缓冲的福尔马林中，并包埋在石蜡中，以提供切片进行组织学评估（动物使用伦理委员会编号4362180119）。在用苏木精和曙红染色的切片中评估结肠炎的严重程度。诸如炎症程度，再生和隐窝损害等参数根据Dieleman等人的方法分级。通过单向方差分析（ANOVA）进行统计分析，然后使用Graph Pad Prism（6.0版）进行Tukey post hoc测试。

结果

MC提取物治疗显着降低了DSS诱发的结肠炎的严重程度，这由临床症状的减轻所证实（p <0.0001）。用MC提取物治疗的大鼠腹泻发作和直肠出血减少。我们观察到DSS治疗组和对照组之间结肠组织的组织病理学变化，但无统计学意义。MC提取物的处理减少了对结肠组织的损害，炎症细胞减少，溃疡形成和杯状细胞保存减少证明了这一点。

结论

MC提取物可减轻临床症状，并提示对DSS诱发的结肠炎具有潜在的抗炎作用。