A nuclear-localized fluorescent light-up probe, NucFP-NO2, was designed and synthesized that can detect CO selectively in an aqueous buffer (pH 7.4, 37 °C) through the CO-mediated transformation of the nitro group into an amino-functionalized moiety. This probe triggered a more than 55-fold "turn-on" fluorescence response to CO without using any metal ions, e.g., Pd, Rh, Fe, etc. The enhanced response is highly selective over a variety of relevant reactive oxygen, nitrogen, and sulfur species and also various biologically important cationic, anionic, and neutral species. The detection limit of this probe for CO is as low as 0.18 μM with a linear range of 0-70 μM. Also, this fluorogenic probe is an efficient candidate for monitoring intracellular CO in living cells (RAW 264.7, A549 cells), and the fluorescence signals predominantly localize in the nuclear region.

中文翻译：

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核定位的基于萘二甲酰亚胺的荧光发光探针，用于选择性检测活细胞中的一氧化碳。

设计并合成了一种核定位的荧光发光探针NucFP-NO2，该探针可以通过将CO介导的硝基基团转化为氨基官能团，选择性地检测水性缓冲液（pH 7.4，37°C）中的CO部分。该探针在不使用任何金属离子（例如Pd，Rh，Fe等）的情况下，触发了对CO的超过55倍的“开启”荧光响应。增强的响应对各种相关的活性氧，氮，和硫物种，以及各种生物学上重要的阳离子，阴离子和中性物种。该探针对CO的检出限低至0.18μM，线性范围为0-70μM。而且，这种荧光探针是监测活细胞（RAW 264.7，A549细胞）中细胞内CO的有效候选者，