Objective Recent evidence points to the gut microbiome’s involvement in postoperative outcomes, including after gastrectomy. Here, we investigated the influence of gastrectomy for gastric cancer on the gut microbiome and metabolome, and how it related to postgastrectomy conditions. Design We performed shotgun metagenomics sequencing and capillary electrophoresis time-of-flight mass spectrometry-based metabolomics analyses on faecal samples collected from participants with a history of gastrectomy for gastric cancer (n=50) and compared them with control participants (n=56). Results The gut microbiota in the gastrectomy group showed higher species diversity and richness (p<0.05), together with greater abundance of aerobes, facultative anaerobes and oral microbes. Moreover, bile acids such as genotoxic deoxycholic acid and branched-chain amino acids were differentially abundant between the two groups (linear discriminant analysis (LDA) effect size (LEfSe): p<0.05, q<0.1, LDA>2.0), as were also Kyoto Encyclopedia of Genes and Genomes modules involved in nutrient transport and organic compounds biosynthesis (LEfSe: p<0.05, q<0.1, LDA>2.0). Conclusion Our results reveal alterations of gut microbiota after gastrectomy, suggesting its association with postoperative comorbidities. The multi-omic approach applied in this study could complement the follow-up of patients after gastrectomy.

中文翻译：

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胃切除术治疗胃癌对粪便微生物组和代谢组谱的影响

目的最近的证据表明肠道微生物组参与术后结果，包括胃切除术后。在这里，我们研究了胃癌切除术对肠道微生物组和代谢组的影响，以及它与胃切除术后状况的关系。设计 我们对从有胃癌切除史的参与者 (n=50) 收集的粪便样本进行了鸟枪宏基因组学测序和基于毛细管电泳飞行时间质谱的代谢组学分析，并将它们与对照参与者 (n=56) 进行了比较. 结果胃切除组肠道菌群的物种多样性和丰富度更高（p<0.05），需氧菌、兼性厌氧菌和口腔微生物的丰度更高。而且，胆汁酸如遗传毒性脱氧胆酸和支链氨基酸在两组之间差异丰富（线性判别分析（LDA）效应大小（LEfSe）：p<0.05，q<0.1，LDA>2.0），京都也是如此涉及营养运输和有机化合物生物合成的基因和基因组模块百科全书（LEfSe：p<0.05，q<0.1，LDA>2.0）。结论我们的结果揭示了胃切除术后肠道微生物群的改变，表明其与术后合并症有关。本研究中应用的多组学方法可以补充胃切除术后患者的随访。京都基因百科全书和基因组模块也涉及营养运输和有机化合物生物合成（LEfSe：p<0.05，q<0.1，LDA>2.0）。结论我们的结果揭示了胃切除术后肠道微生物群的改变，表明其与术后合并症有关。本研究中应用的多组学方法可以补充胃切除术后患者的随访。京都基因百科全书和基因组模块也涉及营养运输和有机化合物生物合成（LEfSe：p<0.05，q<0.1，LDA>2.0）。结论我们的结果揭示了胃切除术后肠道微生物群的改变，表明其与术后合并症有关。本研究中应用的多组学方法可以补充胃切除术后患者的随访。