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Effect of morphine exposure on novel object memory of the offspring: The role of histone H3 and ΔFosB.
Brain Research Bulletin ( IF 3.8 ) Pub Date : 2020-01-17 , DOI: 10.1016/j.brainresbull.2020.01.011 Mitra-Sadat Sadat-Shirazi 1 , Pardis Asgari 1 , Sarah Mahboubi 2 , Setareh Nouri Zadeh-Tehrani 1 , Ghorbangol Ashabi 3 , Kiyana Rohbani 4 , Saba Sabzevari 4 , Haniyeh Soltani 1 , Solmaz Khalifeh 5 , Mohammad-Reza Zarrindast 6
Brain Research Bulletin ( IF 3.8 ) Pub Date : 2020-01-17 , DOI: 10.1016/j.brainresbull.2020.01.011 Mitra-Sadat Sadat-Shirazi 1 , Pardis Asgari 1 , Sarah Mahboubi 2 , Setareh Nouri Zadeh-Tehrani 1 , Ghorbangol Ashabi 3 , Kiyana Rohbani 4 , Saba Sabzevari 4 , Haniyeh Soltani 1 , Solmaz Khalifeh 5 , Mohammad-Reza Zarrindast 6
Affiliation
It has been demonstrated that alteration in histone acetylation in the regions of the brain involved in the reward which may have an important role in morphine addiction. It is well established that epigenetic changes prior to birth influence the function and development of the brain. The current study was designed to evaluate changes in novel object memory, histone acetylation and ΔFosB in the brain of the offspring of morphine-withdrawn parents. Male and female Wistar rats received morphine orally for 21 following days. After ten days of abstinent, they were prepared for mating. The male offspring of the first parturition were euthanized on postnatal days 5, 21, 30 and 60. The novel object recognition (NOR) test was performed on adult male offspring. The amount of acetylated histone H3 and ΔFosB were evaluated in the prefrontal cortex (PFC) and hippocampus using western blotting. Obtained results indicated that the discrimination index in the NOR test was decreased in the offspring of morphine-withdrawn parents as compared with morphine-naïve offspring. In addition, the level of acetylated histone H3 was decreased in the PFC and hippocampus in the offspring of morphine-withdrawn parents during lifetime (postnatal days 5, 21, 30 and 60). In the case of ΔFosB, it also decreased in these regions in the morphine-withdrawn offspring. These results demonstrated that parental morphine exposure affects NOR memory, and decreased the level of histone H3 acetylation and ΔFosB in the PFC and hippocampus. Taken together, the effect of morphine might be transmitted to the next generation even after stop consuming morphine.
中文翻译：
吗啡暴露对后代新物体记忆的影响：组蛋白 H3 和 ΔFosB 的作用。
已经证明，参与奖励的大脑区域中组蛋白乙酰化的改变可能在吗啡成瘾中起重要作用。众所周知，出生前的表观遗传变化会影响大脑的功能和发育。目前的研究旨在评估吗啡戒断父母后代大脑中新物体记忆、组蛋白乙酰化和 ΔFosB 的变化。雄性和雌性 Wistar 大鼠在接下来的 21 天内口服吗啡。禁欲十天后，他们准备交配。第一次分娩的雄性后代在出生后第 5、21、30 和 60 天被安乐死。对成年雄性后代进行了新物体识别 (NOR) 测试。使用蛋白质印迹法评估前额叶皮层 (PFC) 和海马中乙酰化组蛋白 H3 和 ΔFosB 的量。获得的结果表明，与吗啡幼稚后代相比，吗啡戒断父母的后代在 NOR 测试中的辨别指数降低。此外，在一生中（出生后第 5、21、30 和 60 天），停用吗啡的父母的后代 PFC 和海马中乙酰化组蛋白 H3 的水平降低。在 ΔFosB 的情况下，它也在吗啡戒断后代的这些区域中减少。这些结果表明，父母的吗啡暴露会影响 NOR 记忆，并降低 PFC 和海马中组蛋白 H3 乙酰化和 ΔFosB 的水平。综合起来，
更新日期：2020-01-17
中文翻译：
吗啡暴露对后代新物体记忆的影响：组蛋白 H3 和 ΔFosB 的作用。
已经证明，参与奖励的大脑区域中组蛋白乙酰化的改变可能在吗啡成瘾中起重要作用。众所周知，出生前的表观遗传变化会影响大脑的功能和发育。目前的研究旨在评估吗啡戒断父母后代大脑中新物体记忆、组蛋白乙酰化和 ΔFosB 的变化。雄性和雌性 Wistar 大鼠在接下来的 21 天内口服吗啡。禁欲十天后，他们准备交配。第一次分娩的雄性后代在出生后第 5、21、30 和 60 天被安乐死。对成年雄性后代进行了新物体识别 (NOR) 测试。使用蛋白质印迹法评估前额叶皮层 (PFC) 和海马中乙酰化组蛋白 H3 和 ΔFosB 的量。获得的结果表明，与吗啡幼稚后代相比，吗啡戒断父母的后代在 NOR 测试中的辨别指数降低。此外，在一生中（出生后第 5、21、30 和 60 天），停用吗啡的父母的后代 PFC 和海马中乙酰化组蛋白 H3 的水平降低。在 ΔFosB 的情况下，它也在吗啡戒断后代的这些区域中减少。这些结果表明，父母的吗啡暴露会影响 NOR 记忆，并降低 PFC 和海马中组蛋白 H3 乙酰化和 ΔFosB 的水平。综合起来，
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